Pseudomonas aeruginosa PAO1, PA3533 (grxD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3533
Name
grxD
Synonym: ydhD
Replicon chromosome
Genomic location 3952061 - 3952387 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252223.1
GI 15598729
Affymetrix PA3533_at
DNASU PaCD00007554
Entrez 880011
GenBank AAG06921.1
INSDC AAG06921.1
NCBI Locus Tag PA3533
protein_id(GenBank) gb|AAG06921.1|AE004773_10|gnl|PseudoCAP|PA3533
TIGR NTL03PA03533
UniParc UPI00000C5A59
UniProtKB Acc Q9HY77
UniProtKB ID Q9HY77_PSEAE
UniRef100 UniRef100_W0YTI0
UniRef50 UniRef50_Q4K742
UniRef90 UniRef90_S6AYX6

Product

Feature Type CDS
Coding Frame 1
Product Name
GrxD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) -3.08
Kyte-Doolittle Hydrophobicity Value -0.077
Molecular Weight (kDa) 11.8
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 275 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002132 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3533
Search term: grxD
Search term: GrxD

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutaredoxin 3 [Source:HGNC Symbol;Acc:HGNC:15987]
E-value: 3.5e-19
Percent Identity: 41.7
Ensembl 110, assembly GRCh38.p14
glutaredoxin 3 [Source:HGNC Symbol;Acc:HGNC:15987]
E-value: 3.5e-19
Percent Identity: 41.7
Ensembl 110, assembly GRCh38.p14
glutaredoxin 3 [Source:HGNC Symbol;Acc:HGNC:15987]
E-value: 3.5e-19
Percent Identity: 41.7

References

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Mutation of the gene encoding monothiol glutaredoxin (GrxD) in Pseudomonas aeruginosa increases its susceptibility to polymyxins.
Romsang A, Leesukon P, Duangnkern J, Vattanaviboon P, Mongkolsuk S
Int J Antimicrob Agents 2015 Mar;45(3):314-8
PubMed ID: 25593012