Pseudomonas aeruginosa PAO1, PA3541 (alg8)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3541
Name
alg8
Replicon chromosome
Genomic location 3964275 - 3965759 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252231.1
GI 15598737
Affymetrix PA3541_at
Entrez 879005
GenBank AAG06929.1
INSDC AAG06929.1
NCBI Locus Tag PA3541
protein_id(GenBank) gb|AAG06929.1|AE004774_7|gnl|PseudoCAP|PA3541
TIGR NTL03PA03541
UniParc UPI00000C5A5F
UniProtKB Acc Q52463
UniProtKB ID ALG8_PSEAE
UniRef100 UniRef100_Q52463
UniRef50 UniRef50_Q52463
UniRef90 UniRef90_Q52463

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate biosynthesis protein Alg8
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 22.55
Kyte-Doolittle Hydrophobicity Value 0.216
Molecular Weight (kDa) 56.5
Isoelectric Point (pI) 10.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 10 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002125 (492 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3541
Search term: alg8

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
Maharaj R, May TB, Wang SK, Chakrabarty AM
Gene 1993 Dec 22;136(1-2):267-9
PubMed ID: 8294014
The Azotobacter vinelandii alg8 and alg44 genes are essential for alginate synthesis and can be transcribed from an algD-independent promoter.
Mejía-Ruíz H, Guzmán J, Moreno S, Soberón-Chávez G, Espín G
Gene 1997 Oct 15;199(1-2):271-7
PubMed ID: 9358065