Pseudomonas aeruginosa PAO1, PA3547 (algL)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA3547
|
| Name |
algL
|
| Replicon | chromosome |
| Genomic location | 3973014 - 3974117 (+ strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
| Comment | AlgL prevents accumulation of alginate within the periplasmic space |
| Comment | AlgL is required for cell viability and maintaining membrane integrity during alginate production |
Cross-References
| RefSeq | NP_252237.1 |
| GI | 15598743 |
| Affymetrix | PA3547_algL_at |
| Entrez | 878552 |
| GenBank | AAG06935.1 |
| INSDC | AAG06935.1 |
| NCBI Locus Tag | PA3547 |
| protein_id(GenBank) | gb|AAG06935.1|AE004775_6|gnl|PseudoCAP|PA3547 |
| TIGR | NTL03PA03547 |
| UniParc | UPI0000125852 |
| UniProtKB Acc | Q06749 |
| UniProtKB ID | ALGL_PSEAE |
| UniRef100 | UniRef100_Q02R18 |
| UniRef50 | UniRef50_Q9L7P2 |
| UniRef90 | UniRef90_Q02R18 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
poly(beta-d-mannuronate) lyase precursor AlgL
|
| Synonyms |
Alginate lyase (AlgL) |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 5.33 |
| Kyte-Doolittle Hydrophobicity Value | -0.449 |
| Molecular Weight (kDa) | 40.8 |
| Isoelectric Point (pI) | 8.79 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4OZW | LYASE | 02/19/14 | Crystal Structure of the periplasmic alginate lyase AlgL H202A mutant | Pseudomonas aeruginosa | 1.64 | X-RAY DIFFRACTION | 99.7 |
| 7SA8 | LYASE | 09/22/21 | Crystal Structure of the periplasmic lyase AlgL K66A Mutant | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 99.7 |
| 4OZV | LYASE | 02/19/14 | Crystal Structure of the periplasmic alginate lyase AlgL | Pseudomonas aeruginosa | 1.642 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0091 |
| Category | Alginate biosynthesis |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 12 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG002119 (490 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA3547
Search term: algL
Search term: poly(beta-d-mannuronate) lyase precursor AlgL
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.
Schiller NL, Monday SR, Boyd CM, Keen NT, Ohman DE
J Bacteriol 1993 Aug;175(15):4780-9
PubMed ID: 8335634
|
|
Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product.
Boyd A, Ghosh M, May TB, Shinabarger D, Keogh R, Chakrabarty AM
Gene 1993 Sep 6;131(1):1-8
PubMed ID: 8370530
|
|
The Pseudomonas aeruginosa homeostasis enzyme AlgL clears the periplasmic space of accumulated alginate during polymer biosynthesis.
Gheorghita AA, Wolfram F, Whitfield GB, Jacobs HM, Pfoh R, Wong SSY, Guitor AK, Goodyear MC, Berezuk AM, Khursigara CM, Parsek MR, Howell PL
J Biol Chem 2022 Feb;298(2):101560
PubMed ID: 34990713
|