Pseudomonas aeruginosa PAO1, PA3673 (plsB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3673
Name
plsB
Replicon chromosome
Genomic location 4112285 - 4114789 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252363.1
GI 15598869
Affymetrix PA3673_plsB_at
Entrez 880591
GenBank AAG07061.1
INSDC AAG07061.1
NCBI Locus Tag PA3673
protein_id(GenBank) gb|AAG07061.1|AE004787_1|gnl|PseudoCAP|PA3673
TIGR NTL03PA03673
UniParc UPI0000131C37
UniProtKB Acc Q9HXW7
UniProtKB ID PLSB_PSEAE
UniRef100 UniRef100_B7V2U0
UniRef50 UniRef50_Q886Q7
UniRef90 UniRef90_B7V2U0

Product

Feature Type CDS
Coding Frame 1
Product Name
glycerol-3-phosphate acyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (94.82 kDa, PMID:21751344)
Charge (pH 7) 18.00
Kyte-Doolittle Hydrophobicity Value -0.097
Molecular Weight (kDa) 94.8
Isoelectric Point (pI) 9.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 105 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002001 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3673
Search term: plsB

Human Homologs

Ensembl 110, assembly GRCh38.p14
glyceronephosphate O-acyltransferase [Source:HGNC Symbol;Acc:HGNC:4416]
E-value: 2.2e-44
Percent Identity: 29.4

References

Partial NH2- and COOH-terminal sequence and cyanogen bromide peptide analysis of Escherichia coli sn-glycerol-3-phosphate acyltransferase.
Green PR, Vanaman TC, Modrich P, Bell RM
J Biol Chem 1983 Sep 25;258(18):10862-6
PubMed ID: 6350296
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344