Pseudomonas aeruginosa PAO1, PA3724 (lasB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3724
Name
lasB
Replicon chromosome
Genomic location 4168987 - 4170483 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252413.1
GI 15598919
Affymetrix PA3724_lasB_at
Entrez 880368
GenBank AAG07111.1
NCBI Locus Tag PA3724
protein_id(GenBank) gb|AAG07111.1|AE004791_8|gnl|PseudoCAP|PA3724
TIGR NTL03PA03724
UniProtKB Acc P14756
UniProtKB ID ELAS_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
elastase LasB
Product Name Confidence: Class 1
Synonyms neutral metalloproteinase
pseudolysin precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.48
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 53.7
Isoelectric Point (pI) 6.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8CR3 HYDROLASE 03/07/23 Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa PAO1 1.12 X-RAY DIFFRACTION 98.4
8CR7 HYDROLASE 03/07/23 Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7 Pseudomonas aeruginosa PA7 1.5 X-RAY DIFFRACTION 95.9
8CR4 HYDROLASE 03/07/23 Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816 Pseudomonas aeruginosa 0.91 X-RAY DIFFRACTION 94.9
7NLK HYDROLASE 02/22/21 LasB, N-aryl-2-butylmercaptoacetamide Pseudomonas aeruginosa (strain UCBPP-PA14) 1.7 X-RAY DIFFRACTION 98.7
1EZM HYDROLASE 01/13/92 THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0
6FZX HYDROLASE 03/15/18 LasB, hydroxymate Inhibitor Complex Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
7OC7 HYDROLASE 04/26/21 LasB, alpha-alkyl-N-aryl mercaptoacetamide Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 98.7
6F8B HYDROLASE 12/12/17 LasB bound to thiol based inhibitor Pseudomonas aeruginosa 1.3 X-RAY DIFFRACTION 100.0
7NLM HYDROLASE 02/22/21 LasB, N-aryl-2-butylmercaptoacetamide Pseudomonas aeruginosa (strain UCBPP-PA14) 1.65 X-RAY DIFFRACTION 98.7
7QH1 HYDROLASE 12/10/21 Discovery and development of a novel inhaled antivirulence therapy for the treatment of Pseudomonas aeruginosa infections in patients with chronic respiratory disease Pseudomonas aeruginosa 2.74 X-RAY DIFFRACTION 100.0
4K89 HYDROLASE 04/18/13 Crystal structure of Pseudomonas aeruginosa strain K solvent tolerant elastase Pseudomonas aeruginosa 1.39 X-RAY DIFFRACTION 99.7
7AJR HYDROLASE 09/29/20 Virtual screening approach leading to the identification of a novel and tractable series of Pseudomonas aeruginosa elastase inhibitors Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 100.0
1U4G HYDROLASE 07/25/04 Elastase of Pseudomonas aeruginosa with an inhibitor Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
3DBK HYDROLASE 06/01/08 Pseudomonas aeruginosa elastase with phosphoramidon Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-(1-Carboxy-3-Phenylpropyl)Phenylalanyl-Alpha-Asparagine DB02307 DrugBank 5.1.4 P14756 Elastase 100.0 498 6.5e-289

Virulence Evidence

Source 1
Database PseudoCAP
Category Elastase
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Oryctolagus cuniculus
Notes LasB mutation reduced invasion of rabbit corneal epithelial (RCE) cells by 70%
Reference 12904569
Source 2
Database VFDB : VF0087
Category Elastase
Evidence
Host Organism
Notes
Reference
Source 3
Database PseudoCAP
Category Elastase
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Cavia porcellus
Notes
Infection model Guinea pig acute lung infection
Reference 6401692

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 114 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001959 (310 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3724
Search term: lasB
Search term: elastase LasB

Human Homologs

References

Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity.
Fricke B, Parchmann O, Kruse K, Rücknagel P, Schierhorn A, Menge S
Biochim Biophys Acta 1999 Aug 30;1454(3):236-50
PubMed ID: 10452958
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene.
Bever RA, Iglewski BH
J Bacteriol 1988 Sep;170(9):4309-14
PubMed ID: 2842313
Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution.
Thayer MM, Flaherty KM, McKay DB
J Biol Chem 1991 Feb 15;266(5):2864-71
PubMed ID: 1899664
Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.
Olson JC, Ohman DE
J Bacteriol 1992 Jun;174(12):4140-7
PubMed ID: 1597429
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203