Pseudomonas aeruginosa PAO1, PA3751 (purT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3751
Name
purT
Replicon chromosome
Genomic location 4203324 - 4204505 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252440.1
GI 15598946
Affymetrix PA3751_purT_at
Entrez 880455
GenBank AAG07138.1
INSDC AAG07138.1
NCBI Locus Tag PA3751
protein_id(GenBank) gb|AAG07138.1|AE004794_3|gnl|PseudoCAP|PA3751
TIGR NTL03PA03751
UniParc UPI00000C5AF2
UniProtKB Acc Q9HXP3
UniProtKB ID PURT_PSEAE
UniRef100 UniRef100_B7UYK0
UniRef50 UniRef50_P39771
UniRef90 UniRef90_B7UYK0

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylglycinamide formyltransferase 2
Product Name Confidence: Class 2
Synonyms glycinamide ribonucleotide transformylase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.94
Kyte-Doolittle Hydrophobicity Value -0.048
Molecular Weight (kDa) 42.3
Isoelectric Point (pI) 6.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 519 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001931 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3751
Search term: purT

Human Homologs

References

Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli.
Marolewski A, Smith JM, Benkovic SJ
Biochemistry 1994 Mar 8;33(9):2531-7
PubMed ID: 8117714
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994