Pseudomonas aeruginosa PAO1, PA3770 (guaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3770
Name
guaB
Replicon chromosome
Genomic location 4227317 - 4228786 (- strand)

Cross-References

RefSeq NP_252459.1
GI 15598965
Affymetrix PA3770_guaB_at
Entrez 880535
GenBank AAG07157.1
INSDC AAG07157.1
NCBI Locus Tag PA3770
protein_id(GenBank) gb|AAG07157.1|AE004796_2|gnl|PseudoCAP|PA3770
TIGR NTL03PA03770
UniParc UPI00000C5B02
UniProtKB Acc Q9HXM5
UniProtKB ID Q9HXM5_PSEAE
UniRef100 UniRef100_W0YYK6
UniRef50 UniRef50_P21879
UniRef90 UniRef90_S6AG33

Product

Feature Type CDS
Coding Frame 1
Product Name
inosine-5'-monophosphate dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.36
Kyte-Doolittle Hydrophobicity Value 0.027
Molecular Weight (kDa) 51.7
Isoelectric Point (pI) 6.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6GK9 OXIDOREDUCTASE 05/18/18 Inhibited structure of IMPDH from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.54 X-RAY DIFFRACTION 100.0
3ZFH OXIDOREDUCTASE 12/11/12 Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase PSEUDOMONAS AERUGINOSA PAO1 2.25 X-RAY DIFFRACTION 100.0
4DQW OXIDOREDUCTASE 02/16/12 Crystal Structure Analysis of PA3770 Pseudomonas aeruginosa 2.51 X-RAY DIFFRACTION 100.0
7PJI OXIDOREDUCTASE 08/24/21 Crystal structure of Pseudomonas aeruginosa guaB (IMP dehydrogenase) bound to ATP and GDP at 1.65A resolution Pseudomonas aeruginosa 1.65 X-RAY DIFFRACTION 100.0
6GJV OXIDOREDUCTASE 05/17/18 apo-structure of IMPDH from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.11 X-RAY DIFFRACTION 100.0
4AVF OXIDOREDUCTASE 05/25/12 Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase PSEUDOMONAS AERUGINOSA 2.23 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 631 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001893 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3770
Search term: guaB

Human Homologs

Ensembl 110, assembly GRCh38.p14
inosine monophosphate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6052]
E-value: 5.9e-90
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
inosine monophosphate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6052]
E-value: 5.9e-90
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
inosine monophosphate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6052]
E-value: 5.9e-90
Percent Identity: 42.1

References

Nucleotide sequence of the guaB locus encoding IMP dehydrogenase of Escherichia coli K12.
Tiedeman AA, Smith JM
Nucleic Acids Res 1985 Feb 25;13(4):1303-16
PubMed ID: 2860637
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994