Pseudomonas aeruginosa PAO1, PA3825

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3825
Name
Replicon chromosome
Genomic location 4282078 - 4283658 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252514.1
GI 15599020
Affymetrix PA3825_at
Entrez 879846
GenBank AAG07212.1
INSDC AAG07212.1
NCBI Locus Tag PA3825
protein_id(GenBank) gb|AAG07212.1|AE004800_1|gnl|PseudoCAP|PA3825
TIGR NTL03PA03825
UniParc UPI00000C5B2A
UniProtKB Acc Q9HXH7
UniProtKB ID Q9HXH7_PSEAE
UniRef100 UniRef100_Q9HXH7
UniRef50 UniRef50_A0A0B7DC83
UniRef90 UniRef90_Q9HXH7

Product

Feature Type CDS
Coding Frame 1
Product Name
Cyclic-guanylate-specific phosphodiesterase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -6.75
Kyte-Doolittle Hydrophobicity Value 0.032
Molecular Weight (kDa) 58.3
Isoelectric Point (pI) 5.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5MF5 HYDROLASE 11/17/16 PA3825-EAL Mg-CdG Structure Pseudomonas aeruginosa 1.77 X-RAY DIFFRACTION 99.6
5MKG HYDROLASE 12/04/16 PA3825-EAL Ca-CdG Structure Pseudomonas aeruginosa 2.44 X-RAY DIFFRACTION 100.0
4Y9O UNKNOWN FUNCTION 02/17/15 PA3825-EAL Metal-Free-Apo Structure - Manganese Co-crystallisation Pseudomonas aeruginosa PAO1 1.81 X-RAY DIFFRACTION 99.6
4Y9P SIGNALING PROTEIN 02/17/15 PA3825-EAL Ca-CdG Structure Pseudomonas aeruginosa PAO1 2.44 X-RAY DIFFRACTION 100.0
4Y8E METAL BINDING PROTEIN 02/16/15 PA3825-EAL Ca-Apo Structure Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.61 X-RAY DIFFRACTION 100.0
4Y9N UNKNOWN FUNCTION 02/17/15 PA3825-EAL Metal-Free-Apo Structure - Magnesium Co-crystallisation Pseudomonas aeruginosa PAO1 1.92 X-RAY DIFFRACTION 100.0
4Y9M UNKNOWN FUNCTION 02/17/15 PA3825-EAL Metal-Free-Apo Structure Pseudomonas aeruginosa PAO1 1.6 X-RAY DIFFRACTION 100.0
5MFU HYDROLASE 11/18/16 PA3825-EAL Mn-pGpG Structure Pseudomonas aeruginosa 2.15 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001840 (462 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3825

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2839-44
PubMed ID: 16477007