Pseudomonas aeruginosa PAO1, PA3878 (narX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3878
Name
narX
Replicon chromosome
Genomic location 4345010 - 4346878 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252567.1
GI 15599073
Affymetrix PA3878_narX_at
Entrez 878321
GenBank AAG07265.1
INSDC AAG07265.1
NCBI Locus Tag PA3878
protein_id(GenBank) gb|AAG07265.1|AE004805_3|gnl|PseudoCAP|PA3878
TIGR NTL03PA03878
UniParc UPI00000D426E
UniProtKB Acc G3XD66
UniProtKB ID G3XD66_PSEAE
UniRef100 UniRef100_W0YVS9
UniRef50 UniRef50_Q02S43
UniRef90 UniRef90_Q02S43

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component sensor NarX
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (69.32 kDa, PMID:21751344)
Charge (pH 7) -9.31
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 69.3
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 415 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001793 (373 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3878
Search term: narX

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
Stewart V, Parales J Jr, Merkel SM
J Bacteriol 1989 Apr;171(4):2229-34
PubMed ID: 2649492
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
The OpdQ porin of Pseudomonas aeruginosa is regulated by environmental signals associated with cystic fibrosis including nitrate-induced regulation involving the NarXL two-component system.
Fowler RC, Hanson ND
Microbiologyopen 2015 Dec;4(6):967-82
PubMed ID: 26459101