Pseudomonas aeruginosa PAO1, PA3901 (fecA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3901
Name
fecA
Replicon chromosome
Genomic location 4368837 - 4371191 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252590.1
GI 15599096
Affymetrix PA3901_fecA_at
Entrez 878918
GenBank AAG07288.1
INSDC AAG07288.1
NCBI Locus Tag PA3901
protein_id(GenBank) gb|AAG07288.1|AE004807_7|gnl|PseudoCAP|PA3901
TIGR NTL03PA03901
UniParc UPI00000C5B69
UniProtKB Acc Q9HXB2
UniProtKB ID Q9HXB2_PSEAE
UniRef100 UniRef100_Q9HXB2
UniRef50 UniRef50_P13036
UniRef90 UniRef90_Q9HXB2

Product

Feature Type CDS
Coding Frame 1
Product Name
Fe(III) dicitrate transport protein FecA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (85.47 kDa, PMID:21751344)
Charge (pH 7) -12.66
Kyte-Doolittle Hydrophobicity Value -0.489
Molecular Weight (kDa) 85.5
Isoelectric Point (pI) 5.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 281 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001774 (852 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3901
Search term: fecA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.
Staudenmaier H, Van Hove B, Yaraghi Z, Braun V
J Bacteriol 1989 May;171(5):2626-33
PubMed ID: 2651410
A novel purification of ferric citrate receptor (FecA) from Escherichia coli UT5600 and further characterization of its binding activity.
Zhou XH, van der Helm D
Biometals 1993 Spring;6(1):37-44
PubMed ID: 8471824
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344