Pseudomonas aeruginosa PAO1, PA3908 (tsiT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3908
Name
tsiT
Synonym: type VI immunity for Tox-Rease
Replicon chromosome
Genomic location 4376012 - 4376731 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252597.1
GI 15599103
Affymetrix PA3908_at
Entrez 878965
GenBank AAG07295.1
INSDC AAG07295.1
NCBI Locus Tag PA3908
protein_id(GenBank) gb|AAG07295.1|AE004808_5|gnl|PseudoCAP|PA3908
TIGR NTL03PA03908
UniParc UPI00000C5B6E
UniProtKB Acc Q9HXA5
UniProtKB ID Q9HXA5_PSEAE
UniRef100 UniRef100_Q9HXA5
UniRef50 UniRef50_Q9HXA5
UniRef90 UniRef90_Q9HXA5

Product

Feature Type CDS
Coding Frame 1
Product Name
immunity protein, TsiT
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.13
Kyte-Doolittle Hydrophobicity Value -0.292
Molecular Weight (kDa) 27.3
Isoelectric Point (pI) 5.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JDP ANTITOXIN 02/02/19 the imm52 family protein TsiT (PA3908) from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.601 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 7 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001768 (313 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3908
Search term: tsiT

Human Homologs

References

A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex.
Burkinshaw BJ, Liang X, Wong M, Le ANH, Lam L, Dong TG
Nat Microbiol 2018 May;3(5):632-640
PubMed ID: 29632369