Pseudomonas aeruginosa PAO1, PA3999 (dacC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3999
Name
dacC
Synonym: dacD
Replicon chromosome
Genomic location 4478979 - 4480139 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252688.1
GI 15599194
Affymetrix PA3999_dacC_at
Entrez 878956
GenBank AAG07386.1
INSDC AAG07386.1
NCBI Locus Tag PA3999
protein_id(GenBank) gb|AAG07386.1|AE004817_10|gnl|PseudoCAP|PA3999
TIGR NTL03PA03999
UniParc UPI00000D4283
UniProtKB Acc G3XD74
UniProtKB ID G3XD74_PSEAE
UniRef100 UniRef100_W0YY30
UniRef50 UniRef50_A0A024HC00
UniRef90 UniRef90_A6V0B1

Product

Feature Type CDS
Coding Frame 1
Product Name
D-ala-D-ala-carboxypeptidase
Product Name Confidence: Class 2
Synonyms D-ala-D-ala endopeptidase
penicillin-binding protein 6 precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (42.46 kDa, PMID:21751344)
Charge (pH 7) -1.10
Kyte-Doolittle Hydrophobicity Value -0.117
Molecular Weight (kDa) 42.5
Isoelectric Point (pI) 6.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Periplasmic Class 2
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4K91 HYDROLASE 04/19/13 Crystal structure of Penicillin-Binding Protein 5 (PBP5) from Pseudomonas aeruginosa in apo state Pseudomonas aeruginosa UCBPP-PA14 2.05 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 385 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001684 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3999
Search term: dacC

Human Homologs

References

Domain organization of penicillin-binding protein 5 from Escherichia coli analysed by C-terminal truncation.
van der Linden MP, de Haan L, Keck W
Biochem J 1993 Jan 15;289 ( Pt 2)(Pt 2):593-8
PubMed ID: 8424800
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res 1996 Jun 30;3(3):137-55
PubMed ID: 8905232
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344