Pseudomonas aeruginosa PAO1, PA4031 (ppa)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4031
Name	ppa Synonym: ipyR
Replicon	chromosome
Genomic location	4512549 - 4513076 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1

Cross-References

RefSeq	NP_252720.1
GI	15599226
Affymetrix	PA4031_ppa_at
Entrez	879025
GenBank	AAG07418.1
INSDC	AAG07418.1
NCBI Locus Tag	PA4031
protein_id(GenBank)	gb|AAG07418.1|AE004820_3|gnl|PseudoCAP|PA4031
TIGR	NTL03PA04031
UniParc	UPI000012D84B
UniProtKB Acc	Q9HWZ6
UniProtKB ID	IPYR_PSEAE
UniRef100	UniRef100_Q9HWZ6
UniRef50	UniRef50_Q9HWZ6
UniRef90	UniRef90_Q9HWZ6

Product

Feature Type	CDS
Coding Frame	1
Product Name	inorganic pyrophosphatase Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-6.08
Kyte-Doolittle Hydrophobicity Value	-0.142
Molecular Weight (kDa)	19.4
Isoelectric Point (pI)	4.86

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 1 Extracellular Class 1 mass spectrometry evidence used in manual assertion 25488299 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4XEL	HYDROLASE	12/24/14	Crystal structure of Inorganic pyrophosphatase (PPase) from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	2	X-RAY DIFFRACTION	100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
5-amino-1-(4-chlorophenyl)-1H-pyrazole-4-carbonitrile	DB07291	DrugBank	5.1.4	Q3JUV5	Inorganic pyrophosphatase	62.9	175	3.4e-59
N-(pyridin-3-ylmethyl)aniline	DB06851	DrugBank	5.1.4	Q3JUV5	Inorganic pyrophosphatase	62.9	175	3.4e-59

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 482 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001653 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4031 Search term: ppa Search term: inorganic pyrophosphatase

Human Homologs

References

Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12. Lahti R, Pitkäranta T, Valve E, Ilta I, Kukko-Kalske E, Heinonen J J Bacteriol 1988 Dec;170(12):5901-7 PubMed ID: 2848015

The structure of E.coli soluble inorganic pyrophosphatase at 2.7 A resolution. Kankare J, Neal GS, Salminen T, Glumoff T, Glumhoff T [corrected to Glumoff T], Cooperman BS, Lahti R, Goldman A Protein Eng 1994 Jul;7(7):823-30 PubMed ID: 7971944

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142

Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa. Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M J Bacteriol 2015 Feb 15;197(4):762-73 PubMed ID: 25488299