Pseudomonas aeruginosa PAO1, PA4044 (dxs)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4044
Name
dxs
Replicon chromosome
Genomic location 4526547 - 4528430 (+ strand)

Cross-References

RefSeq NP_252733.1
GI 15599239
Affymetrix PA4044_dxs_at
Entrez 879037
GenBank AAG07431.1
INSDC AAG07431.1
NCBI Locus Tag PA4044
protein_id(GenBank) gb|AAG07431.1|AE004821_4|gnl|PseudoCAP|PA4044
TIGR NTL03PA04044
UniParc UPI00001299EC
UniProtKB Acc Q9KGU7
UniProtKB ID DXS_PSEAE
UniRef100 UniRef100_B7V7R4
UniRef50 UniRef50_Q487D3
UniRef90 UniRef90_B7V7R4

Product

Feature Type CDS
Coding Frame 1
Product Name
1-deoxyxylulose-5-phosphate synthase
Product Name Confidence: Class 2
Synonyms dxp synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.76
Kyte-Doolittle Hydrophobicity Value -0.107
Molecular Weight (kDa) 68.0
Isoelectric Point (pI) 5.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8A5K TRANSFERASE 06/15/22 Structural analysis of 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa and Klebsiella pneumoniae reveals conformational changes upon cofactor binding Pseudomonas aeruginosa LESB58 2.3 X-RAY DIFFRACTION 93.9
8A4D TRANSFERASE 06/10/22 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa with thiamine analog inhibitor Pseudomonas aeruginosa LESB58 2.2 X-RAY DIFFRACTION 93.9
8A45 TRANSFERASE 06/10/22 Structural analysis of 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa with 2-acetyl thiamine diphosphate Pseudomonas aeruginosa LESB58 2 X-RAY DIFFRACTION 93.9
8A29 TRANSFERASE 06/02/22 Apo 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa Pseudomonas aeruginosa LESB58 2.1 X-RAY DIFFRACTION 93.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 617 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001640 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4044
Search term: dxs

Human Homologs

References

Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol.
Sprenger GA, Schörken U, Wiegert T, Grolle S, de Graaf AA, Taylor SV, Begley TP, Bringer-Meyer S, Sahm H
Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12857-62
PubMed ID: 9371765