Pseudomonas aeruginosa PAO1, PA4110 (ampC)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA4110
|
| Name |
ampC
|
| Replicon | chromosome |
| Genomic location | 4594029 - 4595222 (+ strand) |
| Transposon Mutants | 3 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_252799.1 |
| GI | 15599305 |
| Affymetrix | PA4110_ampC_at |
| Entrez | 878149 |
| GenBank | AAG07497.1 |
| INSDC | ACQ82807.1 |
| NCBI Locus Tag | PA4110 |
| protein_id(GenBank) | gb|AAG07497.1|AE004827_6|gnl|PseudoCAP|PA4110 |
| TIGR | NTL03PA04110 |
| UniParc | UPI0000001142 |
| UniProtKB Acc | Q541D8 |
| UniProtKB Acc | P24735 |
| UniProtKB ID | Q541D8_PSEAE |
| UniProtKB ID | Q541D8_PSEAI |
| UniRef100 | UniRef100_P24735 |
| UniRef50 | UniRef50_P85302 |
| UniRef90 | UniRef90_P24735 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
beta-lactamase precursor
|
| Synonyms |
cephalosporinase |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 4.38 |
| Kyte-Doolittle Hydrophobicity Value | -0.290 |
| Molecular Weight (kDa) | 43.4 |
| Isoelectric Point (pI) | 8.82 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AMR gene predictions from CARD database
Identified using the Resistance Gene Identifier (RGI)
| Model Name | Definition | Accession | Model Type (?) | SNP | Cutoff | Bit Score | Percent Identity | CARD Version |
| PDC-1 | PDC-1 is a beta-lactamase found in Pseudomonas aeruginosa. [PMID:19258272] | ARO:3002497 | protein homolog model | Perfect | 807.4 | 100.0 | 3.2.5 |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
| Look for predicted 3D structure in AlphaFold DB: Search |
| Look for predicted 3D structure in AlphaFold DB: Search |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4NK3 | HYDROLASE/HYDROLASE INHIBITOR | 11/12/13 | Amp-c beta-lactamase (pseudomonas aeruginosa) in complex with mk-7655 | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
| 8SDN | HYDROLASE | 04/07/23 | Crystal structure of PDC-3 Y221H beta-lactamase | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 99.5 |
| 6UR3 | ANTIBIOTIC | 10/22/19 | Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors | Pseudomonas aeruginosa | 1.423 | X-RAY DIFFRACTION | 100.0 |
| 6UQT | ANTIBIOTIC | 10/21/19 | Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors | Pseudomonas aeruginosa | 1.25 | X-RAY DIFFRACTION | 100.0 |
| 4OOY | hydrolase/hydrolase inhibitor | 02/04/14 | Avibactam and class C beta-lactamases: mechanism of inhibition, conservation of binding pocket and implications for resistance | Pseudomonas aeruginosa | 1.1 | X-RAY DIFFRACTION | 100.0 |
| 2WZZ | HYDROLASE | 12/03/09 | AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-03 | PSEUDOMONAS AERUGINOSA | 1.57 | X-RAY DIFFRACTION | 100.0 |
| 8SDS | HYDROLASE | 04/07/23 | Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor LP-06 | Pseudomonas aeruginosa | 1.63 | X-RAY DIFFRACTION | 99.5 |
| 6I30 | HYDROLASE | 11/02/18 | Crystal structure of the AmpC from Pseudomonas aeruginosa with 1C | Pseudomonas aeruginosa | 2.21 | X-RAY DIFFRACTION | 100.0 |
| 4GZB | HYDROLASE | 09/06/12 | Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa | 1.79 | X-RAY DIFFRACTION | 100.0 |
| 2WZX | HYDROLASE | 12/03/09 | AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-02 | PSEUDOMONAS AERUGINOSA | 1.4 | X-RAY DIFFRACTION | 100.0 |
| 4HEF | HYDROLASE/HYDROLASE INHIBITOR | 10/03/12 | Structure of avibactam bound to Pseudomonas aeruginosa AmpC | Pseudomonas aeruginosa | 1.86 | X-RAY DIFFRACTION | 99.7 |
| 6UQU | ANTIBIOTIC | 10/21/19 | Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors | Pseudomonas aeruginosa | 1.0902 | X-RAY DIFFRACTION | 100.0 |
| 8SDL | HYDROLASE | 04/07/23 | Crystal structure of PDC-3 beta-lactamase | Pseudomonas aeruginosa | 1.75 | X-RAY DIFFRACTION | 99.7 |
| 3S1Y | Hydrolase/Hydrolase Inhibitor | 05/16/11 | AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with a beta-lactamase inhibitor | Pseudomonas aeruginosa | 1.4 | X-RAY DIFFRACTION | 100.0 |
| 3S22 | Hydrolase/Hydrolase Inhibitor | 05/16/11 | AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with an inhibitor | Pseudomonas aeruginosa | 1.65 | X-RAY DIFFRACTION | 100.0 |
| 8SDT | HYDROLASE | 04/07/23 | Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor S02030 | Pseudomonas aeruginosa | 1.38 | X-RAY DIFFRACTION | 99.7 |
| 4WYY | hydrolase/hydrolase inhibitor | 11/18/14 | Crystal Structure of P. aeruginosa AmpC | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 1.28 | X-RAY DIFFRACTION | 100.0 |
| 6S1S | HYDROLASE | 06/19/19 | Crystal structure of AmpC from Pseudomonas aeruginosa in complex with [3-(2-carboxyvinyl)phenyl]boronic acid] inhibitor | Pseudomonas aeruginosa | 1.78 | X-RAY DIFFRACTION | 100.0 |
| 8SDR | HYDROLASE/HYDROLASE INHIBITOR | 04/07/23 | Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor LP-06 | Pseudomonas aeruginosa | 1.35 | X-RAY DIFFRACTION | 99.7 |
| 8SDV | HYDROLASE | 04/07/23 | Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor S02030 | Pseudomonas aeruginosa | 1.42 | X-RAY DIFFRACTION | 99.5 |
| 4X68 | HYDROLASE/HYDROLASE INHIBITOR | 12/07/14 | Crystal Structure of OP0595 complexed with AmpC | Pseudomonas aeruginosa | 1.68 | X-RAY DIFFRACTION | 100.0 |
| 4WZ4 | hydrolase/hydrolase inhibitor | 11/18/14 | Crystal structure of P. aeruginosa AmpC | Pseudomonas aeruginosa PAO1 | 1.05 | X-RAY DIFFRACTION | 100.0 |
| 6UQS | ANTIBIOTIC | 10/21/19 | Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors | Pseudomonas aeruginosa | 1.37 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001588 (493 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA4110
Search term: ampC
Search term: beta-lactamase precursor
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter.
Lodge J, Busby S, Piddock L
FEMS Microbiol Lett 1993 Aug 1;111(2-3):315-20
PubMed ID: 8405939
|
|
A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.
Bauernfeind A, Wagner S, Jungwirth R, Schneider I, Meyer D
Antimicrob Agents Chemother 1997 Sep;41(9):2041-6
PubMed ID: 9303413
|
|
Potentiation of beta-lactams against Pseudomonas aeruginosa strains by Ro 48-1256, a bridged monobactam inhibitor of AmpC beta-lactamases.
Livermore DM, Chen HY
J Antimicrob Chemother 1997 Sep;40(3):335-43
PubMed ID: 9338484
|