Pseudomonas aeruginosa PAO1, PA4204 (ppgL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4204
Name
ppgL
Replicon chromosome
Genomic location 4704139 - 4705305 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252893.1
GI 15599399
Affymetrix PA4204_at
DNASU PaCD00007826
Entrez 880444
GenBank AAG07591.1
INSDC AAG07591.1
NCBI Locus Tag PA4204
protein_id(GenBank) gb|AAG07591.1|AE004837_2|gnl|PseudoCAP|PA4204
TIGR NTL03PA04204
UniParc UPI00000C5C71
UniProtKB Acc Q9HWH7
UniProtKB ID Q9HWH7_PSEAE
UniRef100 UniRef100_Q9HWH7
UniRef50 UniRef50_A0A0B7D929
UniRef90 UniRef90_Q9HWH7

Product

Feature Type CDS
Coding Frame 1
Product Name
periplasmic gluconolactonase, PpgL
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.61
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 41.6
Isoelectric Point (pI) 7.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IGB HYDROLASE 09/25/18 the structure of Pseudomonas aeruginosa Periplasmic gluconolactonase, PpgL Pseudomonas aeruginosa PAO1 1.651 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 188 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001503 (508 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4204
Search term: ppgL

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The PA4204 gene encodes a periplasmic gluconolactonase (PpgL) which is important for fitness of Pseudomonas aeruginosa.
Tarighi S, Wei Q, Cámara M, Williams P, Fletcher MP, Kajander T, Cornelis P
Microbiology (Reading) 2008 Oct;154(Pt 10):2979-2990
PubMed ID: 18832304
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994