Pseudomonas aeruginosa PAO1, PA4238 (rpoA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4238
Name
rpoA
Replicon chromosome
Genomic location 4754423 - 4755424 (- strand)

Cross-References

RefSeq NP_252928.1
GI 15599434
Affymetrix PA4238_rpoA_at
DNASU PaCD00007837
Entrez 881813
GenBank AAG07626.1
INSDC AAG07626.1
NCBI Locus Tag PA4238
protein_id(GenBank) gb|AAG07626.1|AE004841_4|gnl|PseudoCAP|PA4238
TIGR NTL03PA04239
UniParc UPI00001346AC
UniProtKB Acc O52760
UniProtKB ID RPOA_PSEAE
UniRef100 UniRef100_A6UZL3
UniRef50 UniRef50_Q4K557
UniRef90 UniRef90_Q4K557

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-directed RNA polymerase alpha chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (36.65 kDa, PMID:21751344)
Charge (pH 7) -15.59
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 4.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XL4 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) Pseudomonas aeruginosa PAO1 3.86 ELECTRON MICROSCOPY 100.0
7XL3 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) Pseudomonas aeruginosa PAO1 3.13 ELECTRON MICROSCOPY 100.0
7F0R TRANSCRIPTION/DNA 06/06/21 Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 5.8 ELECTRON MICROSCOPY 100.0
7VF9 TRANSCRIPTION 09/10/21 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes Pseudomonas aeruginosa PAO1 4.04 ELECTRON MICROSCOPY 100.0
7XYA TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loading complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.3 ELECTRON MICROSCOPY 100.0
7XYB TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loaded complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.7 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001472 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4238
Search term: rpoA

Human Homologs

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344