Pseudomonas aeruginosa PAO1, PA4265 (tufA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4265
Name
tufA
Replicon chromosome
Genomic location 4767811 - 4769004 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252955.1
GI 15599461
Affymetrix PA4265_tufA_s_at
Entrez 881718
GenBank AAG07653.1
NCBI Locus Tag PA4265
protein_id(GenBank) gb|AAG07653.1|AE004842_2|gnl|PseudoCAP|PA4265
TIGR NTL03PA04266
UniProtKB Acc P09591
UniProtKB ID EFTU_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
elongation factor Tu
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.75
Kyte-Doolittle Hydrophobicity Value -0.152
Molecular Weight (kDa) 43.4
Isoelectric Point (pI) 5.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZV4 TRANSLATION 05/18/15 Structure of Tse6 in complex with EF-Tu Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 3.504 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001434 (870 members)
Putative Inparalogs 1 predicted inparalogs
elongation factor Tu (PA4277) 100.0% identity

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4265
Search term: tufA

Human Homologs

Ensembl 110, assembly GRCh38.p14
Tu translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12420]
E-value: 6.4e-135
Percent Identity: 55.9

References

The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.
An G, Friesen JD
Gene 1980 Dec;12(1-2):33-9
PubMed ID: 7011904
Kinetic studies on the interactions of Escherichia coli K12 elongation factor Tu with GDP and elongation factor Ts.
Chau V, Romero G, Biltonen RL
J Biol Chem 1981 Jun 10;256(11):5591-6
PubMed ID: 7016856
The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.
Laursen RA, L'Italien JJ, Nagarkatti S, Miller DL
J Biol Chem 1981 Aug 10;256(15):8102-9
PubMed ID: 7021545
The structure of the Escherichia coli EF-Tu.EF-Ts complex at 2.5 A resolution.
Kawashima T, Berthet-Colominas C, Wulff M, Cusack S, Leberman R
Nature 1996 Feb 8;379(6565):511-8
PubMed ID: 8596629