Pseudomonas aeruginosa PAO1, PA4269 (rpoC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4269
Name
rpoC
Replicon chromosome
Genomic location 4772279 - 4776478 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_252959.1
GI 15599465
Affymetrix PA4269_rpoC_at
Entrez 881710
GenBank AAG07657.1
INSDC AAG07657.1
NCBI Locus Tag PA4269
protein_id(GenBank) gb|AAG07657.1|AE004842_6|gnl|PseudoCAP|PA4269
TIGR NTL03PA04270
UniParc UPI000013473C
UniProtKB Acc Q9HWC9
UniProtKB ID RPOC_PSEAE
UniRef100 UniRef100_Q02T86
UniRef50 UniRef50_A7ZUK2
UniRef90 UniRef90_Q4K527

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-directed RNA polymerase beta* chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (154.38 kDa, PMID:21751344)
Charge (pH 7) 1.02
Kyte-Doolittle Hydrophobicity Value -0.230
Molecular Weight (kDa) 154.4
Isoelectric Point (pI) 7.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001442 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4269
Search term: rpoC

Human Homologs

Ensembl 97, assembly GRCh38.p12
RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]
E-value: 2.6e-46
Percent Identity: 24.9
Ensembl 97, assembly GRCh38.p12
RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]
E-value: 2.6e-46
Percent Identity: 24.9
Ensembl 97, assembly GRCh38.p12
RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]
E-value: 2.6e-46
Percent Identity: 24.9
Ensembl 97, assembly GRCh38.p12
RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]
E-value: 2.6e-46
Percent Identity: 24.9

References

The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.
Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Guryev SO, Salomatina IS, Shuvaeva TM, Lipkin VM, Sverdlov ED
Nucleic Acids Res 1982 Jul 10;10(13):4035-44
PubMed ID: 6287430
[Genes coding for RNA polymerase in bacteria. III. The use of modified Sanger's method for sequencing the C-terminal region of rpoB gene, N-terminal region of rpoC gene and intercistron region of RNA polymerase in Pseudomonas putida].
Borodin AM, Danilkovich AV, Chernov IP, Azhikina TL, Rostapshov VM
Bioorg Khim 1988 Sep;14(9):1179-82
PubMed ID: 3219133
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878