Pseudomonas aeruginosa PAO1, PA4270 (rpoB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4270
Name
rpoB
Replicon chromosome
Genomic location 4776544 - 4780617 (- strand)
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_252960.1
GI 15599466
Affymetrix PA4270_rpoB_at
Entrez 881699
GenBank AAG07658.1
INSDC AAA25985.1
NCBI Locus Tag PA4270
protein_id(GenBank) gb|AAG07658.1|AE004842_7|gnl|PseudoCAP|PA4270
TIGR NTL03PA04271
UniParc UPI00001346F1
UniProtKB Acc Q51561
UniProtKB ID RPOB_PSEAE
UniRef100 UniRef100_Q51561
UniRef50 UniRef50_P06173
UniRef90 UniRef90_Q51561

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-directed RNA polymerase beta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (150.84 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.87
Kyte-Doolittle Hydrophobicity Value -0.286
Molecular Weight (kDa) 150.8
Isoelectric Point (pI) 5.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001441 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4270
Search term: rpoB

Human Homologs

References

The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OYu, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED
Eur J Biochem 1981 Jun 1;116(3):621-9
PubMed ID: 6266829
A beta subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase.
Lee J, Kashlev M, Borukhov S, Goldfarb A
Proc Natl Acad Sci U S A 1991 Jul 15;88(14):6018-22
PubMed ID: 2068078
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878