Pseudomonas aeruginosa PAO1, PA4270 (rpoB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4270
Name	rpoB
Replicon	chromosome
Genomic location	4776544 - 4780617 (- strand)
Comment	Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq	NP_252960.1
GI	15599466
Affymetrix	PA4270_rpoB_at
Entrez	881699
GenBank	AAG07658.1
INSDC	AAA25985.1
NCBI Locus Tag	PA4270
protein_id(GenBank)	gb|AAG07658.1|AE004842_7|gnl|PseudoCAP|PA4270
TIGR	NTL03PA04271
UniParc	UPI00001346F1
UniProtKB Acc	Q51561
UniProtKB ID	RPOB_PSEAE
UniRef100	UniRef100_Q51561
UniRef50	UniRef50_P06173
UniRef90	UniRef90_Q51561

Product

Feature Type	CDS
Coding Frame	1
Product Name	DNA-directed RNA polymerase beta chain Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (150.84 kDa, PMID:21751344) Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-19.87
Kyte-Doolittle Hydrophobicity Value	-0.286
Molecular Weight (kDa)	150.8
Isoelectric Point (pI)	5.58

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7XYB	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loaded complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.7	ELECTRON MICROSCOPY	100.0
7XL3	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)	Pseudomonas aeruginosa PAO1	3.13	ELECTRON MICROSCOPY	100.0
7VF9	TRANSCRIPTION	09/10/21	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes	Pseudomonas aeruginosa PAO1	4.04	ELECTRON MICROSCOPY	100.0
7XL4	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)	Pseudomonas aeruginosa PAO1	3.86	ELECTRON MICROSCOPY	100.0
7F0R	TRANSCRIPTION/DNA	06/06/21	Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT	5.8	ELECTRON MICROSCOPY	100.0
7XYA	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loading complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.3	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001441 (533 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4270 Search term: rpoB Search term: DNA-directed RNA polymerase beta chain

Human Homologs

References

The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OYu, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED Eur J Biochem 1981 Jun 1;116(3):621-9 PubMed ID: 6266829

A beta subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase. Lee J, Kashlev M, Borukhov S, Goldfarb A Proc Natl Acad Sci U S A 1991 Jul 15;88(14):6018-22 PubMed ID: 2068078

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix. Toyofuku M, Roschitzki B, Riedel K, Eberl L J Proteome Res 2012 Oct 5;11(10):4906-15 PubMed ID: 22909304

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms. Couto N, Schooling SR, Dutcher JR, Barber J J Proteome Res 2015 Oct 2;14(10):4207-22 PubMed ID: 26303878