Pseudomonas aeruginosa PAO1, PA4310 (pctB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4310
Name
pctB
Replicon chromosome
Genomic location 4837390 - 4839279 (+ strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_253000.1
GI 15599506
Affymetrix PA4310_pctB_at
Entrez 881566
GenBank AAG07698.1
INSDC AAG07698.1
NCBI Locus Tag PA4310
protein_id(GenBank) gb|AAG07698.1|AE004847_5|gnl|PseudoCAP|PA4310
TIGR NTL03PA04311
UniParc UPI00000C5CAE
UniProtKB Acc Q9HW91
UniProtKB ID PCTB_PSEAE
UniRef100 UniRef100_Q9HW91
UniRef50 UniRef50_G3XD24
UniRef90 UniRef90_Q9HW91

Product

Feature Type CDS
Coding Frame 1
Product Name
chemotactic transducer PctB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -21.13
Kyte-Doolittle Hydrophobicity Value -0.108
Molecular Weight (kDa) 68.1
Isoelectric Point (pI) 4.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5LT9 SIGNALING PROTEIN 09/06/16 Ligand binding domain of Pseudomonas aeruginosa PAO1 amino acid chemoreceptors PctB in complex with L-Arg Pseudomonas aeruginosa 3 X-RAY DIFFRACTION 100.0
5LTO SIGNALING PROTEIN 09/07/16 Ligand binding domain of Pseudomonas aeruginosa PAO1 amino acid chemoreceptors PctB in complex with L-Gln Pseudomonas aeruginosa 3.459 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 420 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003908 (941 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4310
Search term: pctB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa.
Taguchi K, Fukutomi H, Kuroda A, Kato J, Ohtake H
Microbiology (Reading) 1997 Oct;143 ( Pt 10):3223-3229
PubMed ID: 9353923