Pseudomonas aeruginosa PAO1, PA4386 (groES)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4386
Name
groES
Synonym: mopB
Replicon chromosome
Genomic location 4917175 - 4917468 (- strand)

Cross-References

RefSeq NP_253076.1
GI 15599582
Affymetrix PA4386_groES_at
DNASU PaCD00007899
Entrez 881349
GenBank AAG07774.1
INSDC AAG07774.1
NCBI Locus Tag PA4386
protein_id(GenBank) gb|AAG07774.1|AE004854_10|gnl|PseudoCAP|PA4386
TIGR NTL03PA04387
UniParc UPI000012763D
UniProtKB Acc P30720
UniProtKB ID CH10_PSEAE
UniRef100 UniRef100_A6VB58
UniRef50 UniRef50_O33499
UniRef90 UniRef90_O33499

Product

Feature Type CDS
Coding Frame 1
Product Name
GroES protein
Product Name Confidence: Class 1
Synonyms 10 kD chaperonin
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.2, molecular weight:10.3, PMID:21674802)
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.77
Kyte-Doolittle Hydrophobicity Value -0.072
Molecular Weight (kDa) 10.3
Isoelectric Point (pI) 4.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003977 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4386
Search term: groES
Search term: GroES protein

Human Homologs

References

Cloning and sequencing of the genes coding for the 10- and 60-kDa heat shock proteins from Pseudomonas aeruginosa and mapping of a species-specific epitope.
Sipos A, Klocke M, Frosch M
Infect Immun 1991 Sep;59(9):3219-26
PubMed ID: 1715325
Cloning and nucleotide sequence comparison of the groE operon of Pseudomonas aeruginosa and Burkholderia cepacia.
Jensen P, Fomsgaard A, Høiby N, Hindersson P
APMIS 1995 Feb;103(2):113-23
PubMed ID: 7538307
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802