Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4400
|
Name |
Synonym: NUDIX hydrolase |
Replicon | chromosome |
Genomic location | 4930748 - 4931695 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Pyocyanin content was increased in a PA4400 mutant [PMID:32419387] |
Comment | Inactivation of PA4400 stimulates pyocyanin production, severely impairs swarming motility, decreases virulence, and increases resistance to piperacillin [PMID:32419387] |
RefSeq | NP_253090.1 |
GI | 15599596 |
Affymetrix | PA4400_at |
Entrez | 881318 |
GenBank | AAG07788.1 |
INSDC | AAG07788.1 |
NCBI Locus Tag | PA4400 |
protein_id(GenBank) | gb|AAG07788.1|AE004855_10|gnl|PseudoCAP|PA4400 |
TIGR | NTL03PA04401 |
UniParc | UPI00000C5CFE |
UniProtKB Acc | Q9HW06 |
UniProtKB ID | Q9HW06_PSEAE |
UniRef100 | UniRef100_S0I3E9 |
UniRef50 | UniRef50_D7I481 |
UniRef90 | UniRef90_A0A077JML3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
probable pyrophosphohydrolase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -10.64 |
Kyte-Doolittle Hydrophobicity Value | -0.169 |
Molecular Weight (kDa) | 34.0 |
Isoelectric Point (pI) | 5.11 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 441 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003990 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4400
Search term: probable pyrophosphohydrolase
|
Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion.
Akiyama M, Horiuchi T, Sekiguchi M
Mol Gen Genet 1987 Jan;206(1):9-16
PubMed ID: 3033442
|
Studies on the mutator gene, mutT of Escherichia coli. Molecular cloning of the gene, purification of the gene product, and identification of a novel nucleoside triphosphatase.
Bhatnagar SK, Bessman MJ
J Biol Chem 1988 Jun 25;263(18):8953-7
PubMed ID: 3288626
|
Characterization of the mutT nucleoside triphosphatase of Escherichia coli.
Bhatnagar SK, Bullions LC, Bessman MJ
J Biol Chem 1991 May 15;266(14):9050-4
PubMed ID: 1851162
|
MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis.
Maki H, Sekiguchi M
Nature 1992 Jan 16;355(6357):273-5
PubMed ID: 1309939
|
A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T
DNA Res 1996 Dec 31;3(6):363-77
PubMed ID: 9097039
|
Characterization of the GO system of Pseudomonas aeruginosa.
Oliver A, Sánchez JM, Blázquez J
FEMS Microbiol Lett 2002 Nov 19;217(1):31-5
PubMed ID: 12445642
|
Individual Nudix hydrolases affect diverse features of Pseudomonas aeruginosa.
Drabinska J, Ziecina M, Modzelan M, Jagura-Burdzy G, Kraszewska E
Microbiologyopen 2020 Aug;9(8):e1052
PubMed ID: 32419387
|