Pseudomonas aeruginosa PAO1, PA4400

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: NUDIX hydrolase
Replicon chromosome
Genomic location 4930748 - 4931695 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentPyocyanin content was increased in a PA4400 mutant [PMID:32419387]
Comment Inactivation of PA4400 stimulates pyocyanin production, severely impairs swarming motility, decreases virulence, and increases resistance to piperacillin [PMID:32419387]


RefSeq NP_253090.1
GI 15599596
Affymetrix PA4400_at
Entrez 881318
GenBank AAG07788.1
INSDC AAG07788.1
NCBI Locus Tag PA4400
protein_id(GenBank) gb|AAG07788.1|AE004855_10|gnl|PseudoCAP|PA4400
UniParc UPI00000C5CFE
UniProtKB Acc Q9HW06
UniRef100 UniRef100_S0I3E9
UniRef50 UniRef50_D7I481
UniRef90 UniRef90_A0A077JML3


Feature Type CDS
Coding Frame 1
Product Name
probable pyrophosphohydrolase
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.64
Kyte-Doolittle Hydrophobicity Value -0.169
Molecular Weight (kDa) 34.0
Isoelectric Point (pI) 5.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 441 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003990 (533 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4400

Human Homologs


Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion.
Akiyama M, Horiuchi T, Sekiguchi M
Mol Gen Genet 1987 Jan;206(1):9-16
PubMed ID: 3033442
Studies on the mutator gene, mutT of Escherichia coli. Molecular cloning of the gene, purification of the gene product, and identification of a novel nucleoside triphosphatase.
Bhatnagar SK, Bessman MJ
J Biol Chem 1988 Jun 25;263(18):8953-7
PubMed ID: 3288626
Characterization of the mutT nucleoside triphosphatase of Escherichia coli.
Bhatnagar SK, Bullions LC, Bessman MJ
J Biol Chem 1991 May 15;266(14):9050-4
PubMed ID: 1851162
MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis.
Maki H, Sekiguchi M
Nature 1992 Jan 16;355(6357):273-5
PubMed ID: 1309939
A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.
Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T
DNA Res 1996 Dec 31;3(6):363-77
PubMed ID: 9097039
Characterization of the GO system of Pseudomonas aeruginosa.
Oliver A, Sánchez JM, Blázquez J
FEMS Microbiol Lett 2002 Nov 19;217(1):31-5
PubMed ID: 12445642
Individual Nudix hydrolases affect diverse features of Pseudomonas aeruginosa.
Drabinska J, Ziecina M, Modzelan M, Jagura-Burdzy G, Kraszewska E
Microbiologyopen 2020 Aug;9(8):e1052
PubMed ID: 32419387