Pseudomonas aeruginosa PAO1, PA4442 (cysN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4442
Name
cysN
Replicon chromosome
Genomic location 4974694 - 4976595 (- strand)
Transposon Mutants 11 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253132.1
GI 15599638
Affymetrix PA4442_cysN_at
Entrez 880979
GenBank AAG07830.1
INSDC AAG07830.1
NCBI Locus Tag PA4442
protein_id(GenBank) gb|AAG07830.1|AE004858_8|gnl|PseudoCAP|PA4442
TIGR NTL03PA04443
UniParc UPI0000128D25
UniProtKB Acc O50274
UniProtKB ID CYSNC_PSEAE
UniRef100 UniRef100_O50274
UniRef50 UniRef50_O50274
UniRef90 UniRef90_O50274

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP sulfurylase GTP-binding subunit/APS kinase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.23
Kyte-Doolittle Hydrophobicity Value -0.223
Molecular Weight (kDa) 69.3
Isoelectric Point (pI) 5.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004031 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4442
Search term: cysN

Human Homologs

Ensembl 97, assembly GRCh38.p12
eukaryotic translation elongation factor 1 alpha 2 [Source:HGNC Symbol;Acc:HGNC:3192]
E-value: 1.1e-39
Percent Identity: 27.3
Ensembl 97, assembly GRCh38.p12
eukaryotic translation elongation factor 1 alpha 2 [Source:HGNC Symbol;Acc:HGNC:3192]
E-value: 1.1e-39
Percent Identity: 27.3

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994