Pseudomonas aeruginosa PAO1, PA4444 (mltB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4444
Name
mltB1
Replicon chromosome
Genomic location 4977869 - 4978972 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253134.1
GI 15599640
Affymetrix PA4444_mltB1_at
Entrez 880971
GenBank AAG07832.1
INSDC AAG07832.1
NCBI Locus Tag PA4444
protein_id(GenBank) gb|AAG07832.1|AE004859_1|gnl|PseudoCAP|PA4444
TIGR NTL03PA04445
UniParc UPI00000C5D14
UniProtKB Acc Q9HVX3
UniProtKB ID Q9HVX3_PSEAE
UniRef100 UniRef100_W0YXJ5
UniRef50 UniRef50_A0A085GP08
UniRef90 UniRef90_W0YXJ5

Product

Feature Type CDS
Coding Frame 1
Product Name
soluble and membrane-bound lytic transglycosylases
Product Name Confidence: Class 1
Synonyms murein hydrolase B
membrane-bound lytic transglycosylase, sMltB, soluble lytic transglycosylases B (SltB)
Evidence for Translation
Charge (pH 7) 15.12
Kyte-Doolittle Hydrophobicity Value -0.407
Molecular Weight (kDa) 41.4
Isoelectric Point (pI) 10.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 233 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004033 (306 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4444
Search term: mltB1

Human Homologs

References

Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
Blackburn NT, Clarke AJ
Biochemistry 2002 Jan 22;41(3):1001-13
PubMed ID: 11790124
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and controlled overexpression of the gene encoding the 35 kDa soluble lytic transglycosylase from Escherichia coli.
Dijkstra AJ, Hermann F, Keck W
FEBS Lett 1995 Jun 12;366(2-3):115-8
PubMed ID: 7789526
Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli.
Ehlert K, Höltje JV, Templin MF
Mol Microbiol 1995 May;16(4):761-8
PubMed ID: 7476170