Pseudomonas aeruginosa PAO1, PA4450 (murA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 4984205 - 4985470 (- strand)
Transposon Mutants 1 transposon mutants in PAO1


RefSeq NP_253140.1
GI 15599646
Affymetrix PA4450_murA_at
Entrez 880969
GenBank AAG07838.1
INSDC AAG07838.1
NCBI Locus Tag PA4450
protein_id(GenBank) gb|AAG07838.1|AE004859_7|gnl|PseudoCAP|PA4450
UniParc UPI000012F91D
UniProtKB Acc Q9HVW7
UniRef100 UniRef100_B7UZY9
UniRef50 UniRef50_P70965
UniRef90 UniRef90_B7UZY9


Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Product Name Confidence: Class 2
Synonyms enoylpyruvate transferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.42
Kyte-Doolittle Hydrophobicity Value 0.152
Molecular Weight (kDa) 44.6
Isoelectric Point (pI) 5.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5BQ2 TRANSFERASE 05/28/15 Crystal structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase (UDP-N-acetylglucosamine enolpyruvyl transferase, EPT) from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004039 (535 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4450
Search term: murA

Human Homologs


UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
Ehrt S, Hillen W
FEMS Microbiol Lett 1994 Apr 1;117(2):137-42
PubMed ID: 8181716
Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin.
Skarzynski T, Mistry A, Wonacott A, Hutchinson SE, Kelly VA, Duncan K
Structure 1996 Dec 15;4(12):1465-74
PubMed ID: 8994972
Studies on the conformational changes in the bacterial cell wall biosynthetic enzyme UDP-N-acetylglucosamine enolpyruvyltransferase (MurA).
Schönbrunn E, Svergun DI, Amrhein N, Koch MH
Eur J Biochem 1998 Apr 15;253(2):406-12
PubMed ID: 9654090
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994