Pseudomonas aeruginosa PAO1, PA4483 (gatA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4483
Name
gatA
Replicon chromosome
Genomic location 5013974 - 5015428 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253173.1
GI 15599679
Affymetrix PA4483_gatA_at
Entrez 881180
GenBank AAG07871.1
INSDC AAG07871.1
NCBI Locus Tag PA4483
protein_id(GenBank) gb|AAG07871.1|AE004862_5|gnl|PseudoCAP|PA4483
TIGR NTL03PA04484
UniParc UPI000012B128
UniProtKB Acc Q9HVT8
UniProtKB ID GATA_PSEAE
UniRef100 UniRef100_Q9HVT8
UniRef50 UniRef50_O06491
UniRef90 UniRef90_Q9HVT8

Product

Feature Type CDS
Coding Frame 1
Product Name
Glu-tRNA(Gln) amidotransferase subunit A
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.67
Kyte-Doolittle Hydrophobicity Value -0.119
Molecular Weight (kDa) 51.9
Isoelectric Point (pI) 5.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WJ3 LIGASE/RNA 09/29/14 Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 3.705 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004072 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4483
Search term: gatA

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutaminyl-tRNA amidotransferase subunit QRSL1 [Source:HGNC Symbol;Acc:HGNC:21020]
E-value: 2.3e-94
Percent Identity: 39.4

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994