Pseudomonas aeruginosa PAO1, PA4528 (pilD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4528
Name
pilD
Synonym: xcpA
Replicon chromosome
Genomic location 5072695 - 5073567 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253218.1
GI 15599724
Affymetrix PA4528_pilD_at
Entrez 877861
GenBank AAG07916.1
NCBI Locus Tag PA4528
protein_id(GenBank) gb|AAG07916.1|AE004867_2|gnl|PseudoCAP|PA4528
TIGR NTL03PA04529
UniProtKB Acc P22610
UniProtKB ID LEP4_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 prepilin peptidase PilD
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilD
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.27
Kyte-Doolittle Hydrophobicity Value 0.637
Molecular Weight (kDa) 31.9
Isoelectric Point (pI) 7.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4208
Category Type IV pili biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575
Source 2
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 366 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004109 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4528
Search term: pilD

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili.
Nunn D, Bergman S, Lory S
J Bacteriol 1990 Jun;172(6):2911-9
PubMed ID: 1971619
Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral inner membrane protein homologous to Klebsiella pneumoniae secretion function protein PulO.
Bally M, Ball G, Badere A, Lazdunski A
J Bacteriol 1991 Jan;173(2):479-86
PubMed ID: 1898929
Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.
Bally M, Filloux A, Akrim M, Ball G, Lazdunski A, Tommassen J
Mol Microbiol 1992 May;6(9):1121-31
PubMed ID: 1588814
Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpR.
Kagami Y, Ratliff M, Surber M, Martinez A, Nunn DN
Mol Microbiol 1998 Jan;27(1):221-33
PubMed ID: 9466269