Pseudomonas aeruginosa PAO1, PA4556 (pilE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4556
Name
pilE
Replicon chromosome
Genomic location 5104514 - 5104939 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentAlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq NP_253246.1
GI 15599752
Affymetrix PA4556_pilE_at
Entrez 878451
GenBank AAG07944.1
INSDC AAG07944.1
NCBI Locus Tag PA4556
protein_id(GenBank) gb|AAG07944.1|AE004869_8|gnl|PseudoCAP|PA4556
TIGR NTL03PA04557
UniParc UPI00000C4EC1
UniProtKB Acc G3XD43
UniProtKB ID G3XD43_PSEAE
UniRef100 UniRef100_G3XD43
UniRef50 UniRef50_G3XD43
UniRef90 UniRef90_G3XD43

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 6.92
Kyte-Doolittle Hydrophobicity Value -0.335
Molecular Weight (kDa) 15.3
Isoelectric Point (pI) 10.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Fimbrial
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4NOA UNKNOWN FUNCTION 11/19/13 Truncated minor pilin PilE from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.25 X-RAY DIFFRACTION 98.1

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IVa pili
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004215 (429 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4556
Search term: pilE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The pilE gene product of Pseudomonas aeruginosa, required for pilus biogenesis, shares amino acid sequence identity with the N-termini of type 4 prepilin proteins.
Russell MA, Darzins A
Mol Microbiol 1994 Sep;13(6):973-85
PubMed ID: 7854130
Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon.
Belete B, Lu H, Wozniak DJ
J Bacteriol 2008 Mar;190(6):2023-30
PubMed ID: 18178737