Pseudomonas aeruginosa PAO1, PA4561 (ribF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4561
Name
ribF
Replicon chromosome
Genomic location 5109806 - 5110744 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253251.1
GI 15599757
Affymetrix PA4561_ribF_at
DNASU PaCD00007975
Entrez 878195
GenBank AAG07949.1
INSDC AAG07949.1
NCBI Locus Tag PA4561
protein_id(GenBank) gb|AAG07949.1|AE004869_13|gnl|PseudoCAP|PA4561
TIGR NTL03PA04562
UniParc UPI00000C5D63
UniProtKB Acc Q9HVM3
UniProtKB ID Q9HVM3_PSEAE
UniRef100 UniRef100_Q9HVM3
UniRef50 UniRef50_P0AG42
UniRef90 UniRef90_Q9HVM3

Product

Feature Type CDS
Coding Frame 1
Product Name
riboflavin kinase/FAD synthase
Product Name Confidence: Class 2
Synonyms flavokinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.26
Kyte-Doolittle Hydrophobicity Value -0.081
Molecular Weight (kDa) 34.3
Isoelectric Point (pI) 9.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004220 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4561
Search term: ribF

Human Homologs

References

Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon.
Kamio Y, Lin CK, Regue M, Wu HC
J Biol Chem 1985 May 10;260(9):5616-20
PubMed ID: 2985604
Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes.
Isaki L, Beers R, Wu HC
J Bacteriol 1990 Nov;172(11):6512-7
PubMed ID: 2121716