Pseudomonas aeruginosa PAO1, PA4616

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4616
Name
Replicon chromosome
Genomic location 5173654 - 5174652 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253306.1
GI 15599812
Affymetrix PA4616_at
DNASU PaCD00008000
Entrez 881133
GenBank AAG08004.1
INSDC AAG08004.1
NCBI Locus Tag PA4616
protein_id(GenBank) gb|AAG08004.1|AE004875_10|gnl|PseudoCAP|PA4616
TIGR NTL03PA04617
UniParc UPI00000C5D8C
UniProtKB Acc Q9HVH5
UniProtKB ID Q9HVH5_PSEAE
UniRef100 UniRef100_Q9HVH5
UniRef50 UniRef50_Q8ECK4
UniRef90 UniRef90_Q9HVH5

Product

Feature Type CDS
Coding Frame 1
Product Name
probable c4-dicarboxylate-binding protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 4.69
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 37.0
Isoelectric Point (pI) 9.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4NF0 TRANSPORT PROTEIN 10/30/13 CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM PSEUDOMONAS AERUGINOSA PAO1 (PA4616), TARGET EFI-510182, WITH BOUND L-Malate Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 241 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004270 (337 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4616

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Purification, characterization and nucleotide sequence of the periplasmic C4-dicarboxylate-binding protein (DctP) from Rhodobacter capsulatus.
Shaw JG, Hamblin MJ, Kelly DJ
Mol Microbiol 1991 Dec;5(12):3055-62
PubMed ID: 1809844
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994