Pseudomonas aeruginosa PAO1, PA4694 (ilvC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4694
Name
ilvC
Replicon chromosome
Genomic location 5272455 - 5273471 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253382.1
GI 15599888
Affymetrix PA4694_ilvC_at
Entrez 881483
GenBank AAG08080.1
INSDC AAG08080.1
NCBI Locus Tag PA4694
protein_id(GenBank) gb|AAG08080.1|AE004883_4|gnl|PseudoCAP|PA4694
TIGR NTL03PA04695
UniParc UPI000012D557
UniProtKB Acc Q9HVA2
UniProtKB ID ILVC_PSEAE
UniRef100 UniRef100_B7V1A6
UniRef50 UniRef50_Q9RU74
UniRef90 UniRef90_Q888N4

Product

Feature Type CDS
Coding Frame 1
Product Name
ketol-acid reductoisomerase
Product Name Confidence: Class 2
Synonyms alpha-keto-beta-hydroxylacil reductoisomerase
acetohydroxy-acid isomeroreductase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.26
Kyte-Doolittle Hydrophobicity Value -0.092
Molecular Weight (kDa) 36.4
Isoelectric Point (pI) 5.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1NP3 OXIDOREDUCTASE 01/17/03 Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0
4XIY OXIDOREDUCTASE 01/07/15 Crystal structure of ketol-acid reductoisomerase from Azotobacter Azotobacter vinelandii 2.5 X-RAY DIFFRACTION 93.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004343 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4694
Search term: ilvC

Human Homologs

References

Mechanism of ketol acid reductoisomerase--steady-state analysis and metal ion requirement.
Chunduru SK, Mrachko GT, Calvo KC
Biochemistry 1989 Jan 24;28(2):486-93
PubMed ID: 2653423
Structure and expression of a cyanobacterial ilvC gene encoding acetohydroxyacid isomeroreductase.
Rieble S, Beale SI
J Bacteriol 1992 Dec;174(24):7910-8
PubMed ID: 1459938
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994