Pseudomonas aeruginosa PAO1, PA4727 (pcnB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4727
Name
pcnB
Replicon chromosome
Genomic location 5309326 - 5310729 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253415.1
GI 15599921
Affymetrix PA4727_pcnB_at
Entrez 881610
GenBank AAG08113.1
INSDC AAG08113.1
NCBI Locus Tag PA4727
protein_id(GenBank) gb|AAG08113.1|AE004886_5|gnl|PseudoCAP|PA4727
TIGR NTL03PA04728
UniParc UPI00000C5DE3
UniProtKB Acc Q9HV72
UniProtKB ID Q9HV72_PSEAE
UniRef100 UniRef100_W0YXG2
UniRef50 UniRef50_L0DXZ0
UniRef90 UniRef90_Q02FU5

Product

Feature Type CDS
Coding Frame 1
Product Name
poly(A) polymerase
Product Name Confidence: Class 2
Synonyms polynucleotide adenylyltransferase
plasmid copy number protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 16.89
Kyte-Doolittle Hydrophobicity Value -0.558
Molecular Weight (kDa) 53.3
Isoelectric Point (pI) 10.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 578 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004374 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4727
Search term: pcnB

Human Homologs

References

A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions.
Masters M, March JB, Oliver IR, Collins JF
Mol Gen Genet 1990 Jan;220(2):341-4
PubMed ID: 1691435
Identification of the gene for an Escherichia coli poly(A) polymerase.
Cao GJ, Sarkar N
Proc Natl Acad Sci U S A 1992 Nov 1;89(21):10380-4
PubMed ID: 1438224