Pseudomonas aeruginosa PAO1, PA4827

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4827
Name
Synonym: nat
Replicon chromosome
Genomic location 5418899 - 5419738 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPA4827 has been cloned, expressed, characterised and crystallised. The crystal structure (PDB 1w4t) shows very similar fold to the nat protein from M. smegmatis, with rmsd of 1.3 Angstroms over 240 equivalent atoms.

Cross-References

RefSeq NP_253514.1
GI 15600020
Affymetrix PA4827_at
Entrez 882169
GenBank AAG08212.1
INSDC AAG08212.1
NCBI Locus Tag PA4827
protein_id(GenBank) gb|AAG08212.1|AE004896_2|gnl|PseudoCAP|PA4827
TIGR NTL03PA04828
UniParc UPI00000C5E28
UniProtKB Acc Q9HUY3
UniProtKB ID Q9HUY3_PSEAE
UniRef100 UniRef100_Q9HUY3
UniRef50 UniRef50_Q9HUY3
UniRef90 UniRef90_Q9HUY3

Product

Feature Type CDS
Coding Frame 1
Product Name
arylamine N-acetyltransferase
Product Name Confidence: Class 1
Synonyms NAT
Evidence for Translation
Charge (pH 7) -5.89
Kyte-Doolittle Hydrophobicity Value -0.121
Molecular Weight (kDa) 31.2
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1W4T TRANSFERASE 07/29/04 X-ray crystallographic structure of Pseudomonas aeruginosa arylamine N-acetyltransferase Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 117 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004459 (336 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4827

Human Homologs

References

Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase.
Westwood IM, Holton SJ, Rodrigues-Lima F, Dupret JM, Bhakta S, Noble ME, Sim E
Biochem J 2005 Jan 15;385(Pt 2):605-12
PubMed ID: 15447630
Involvement of Cys69 residue in the catalytic mechanism of N-hydroxyarylamine O-acetyltransferase of Salmonella typhimurium. Sequence similarity at the amino acid level suggests a common catalytic mechanism of acetyltransferase for S. typhimurium and higher organisms.
Watanabe M, Sofuni T, Nohmi T
J Biol Chem 1992 Apr 25;267(12):8429-36
PubMed ID: 1569093
Cloning and characterization of arylamine N-acetyltransferase genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: increased expression results in isoniazid resistance.
Payton M, Auty R, Delgoda R, Everett M, Sim E
J Bacteriol 1999 Feb;181(4):1343-7
PubMed ID: 9973365