Pseudomonas aeruginosa PAO1, PA4839 (speA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4839
Name
speA
Replicon chromosome
Genomic location 5431381 - 5433291 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253526.1
GI 15600032
Affymetrix PA4839_speA_at
Entrez 879594
GenBank AAG08224.1
INSDC AAG08224.1
NCBI Locus Tag PA4839
protein_id(GenBank) gb|AAG08224.1|AE004897_2|gnl|PseudoCAP|PA4839
TIGR NTL03PA04840
UniParc UPI00000C5E34
UniProtKB Acc Q9HUX1
UniProtKB ID SPEA_PSEAE
UniRef100 UniRef100_Q9HUX1
UniRef50 UniRef50_Q9HUX1
UniRef90 UniRef90_Q9HUX1

Product

Feature Type CDS
Coding Frame 1
Product Name
arginine decarboxylase (ADC)
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -21.10
Kyte-Doolittle Hydrophobicity Value -0.245
Molecular Weight (kDa) 70.7
Isoelectric Point (pI) 5.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 458 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004469 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4839
Search term: speA

Human Homologs

References

Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway.
Nakada Y, Itoh Y
Microbiology (Reading) 2003 Mar;149(Pt 3):707-714
PubMed ID: 12634339
Expression of the cloned genes encoding the putrescine biosynthetic enzymes and methionine adenosyltransferase of Escherichia coli (speA, speB, speC and metK).
Boyle SM, Markham GD, Hafner EW, Wright JM, Tabor H, Tabor CW
Gene 1984 Oct;30(1-3):129-36
PubMed ID: 6392022
Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli.
Moore RC, Boyle SM
J Bacteriol 1990 Aug;172(8):4631-40
PubMed ID: 2198270
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994