Pseudomonas aeruginosa PAO1, PA4890 (desT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4890
Name
desT
Synonym: desT
Synonym: yijC
Replicon chromosome
Genomic location 5486356 - 5486985 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentDesT negatively regulates both desB (PA4888) and PA4889

Cross-References

RefSeq NP_253577.1
GI 15600083
Affymetrix PA4890_at
Entrez 882265
GenBank AAG08275.1
INSDC AAG08275.1
NCBI Locus Tag PA4890
protein_id(GenBank) gb|AAG08275.1|AE004902_3|gnl|PseudoCAP|PA4890
TIGR NTL03PA04891
UniParc UPI00000C5E60
UniProtKB Acc Q9HUS3
UniProtKB ID Q9HUS3_PSEAE
UniRef100 UniRef100_W0Z0X0
UniRef50 UniRef50_Q4KJ31
UniRef90 UniRef90_W0Z0X0

Product

Feature Type CDS
Coding Frame 1
Product Name
DesT
Product Name Confidence: Class 1
Synonyms DesT
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.92
Kyte-Doolittle Hydrophobicity Value 0.024
Molecular Weight (kDa) 22.9
Isoelectric Point (pI) 9.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3LSJ TRANSCRIPTION 02/12/10 Crystal structure of DesT in complex with palmitoyl-CoA Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.5
3LSR TRANSCRIPTION/DNA 02/12/10 Crystal structure of DesT in complex with duplex DNA Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.55 X-RAY DIFFRACTION 99.5
3LSP TRANSCRIPTION/DNA 02/12/10 Crystal Structure of DesT bound to desCB promoter and oleoyl-CoA Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.66 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004514 (507 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4890
Search term: desT
Search term: DesT

Human Homologs

References

Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa.
Zhu K, Choi KH, Schweizer HP, Rock CO, Zhang YM
Mol Microbiol 2006 Apr;60(2):260-73
PubMed ID: 16573679