Pseudomonas aeruginosa PAO1, PA4920 (nadE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 5519711 - 5520538 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1


RefSeq NP_253607.1
GI 15600113
Affymetrix PA4920_nadE_at
DNASU PaCD00008129
Entrez 882213
GenBank AAG08305.1
INSDC AAG08305.1
NCBI Locus Tag PA4920
protein_id(GenBank) gb|AAG08305.1|AE004905_3|gnl|PseudoCAP|PA4920
UniParc UPI000012FCD7
UniProtKB Acc Q9HUP3
UniRef100 UniRef100_B7V1Y3
UniRef50 UniRef50_Q8D3T7
UniRef90 UniRef90_A6VD32


Feature Type CDS
Coding Frame 1
Product Name
NH3-dependent NAD synthetase
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.37
Kyte-Doolittle Hydrophobicity Value -0.193
Molecular Weight (kDa) 29.7
Isoelectric Point (pI) 5.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5F23 LIGASE 12/01/15 Crystal structure of NH(3)-dependent NAD(+) synthetase Pseudomonas aeruginosa in complex with NAD PSEUDOMONAS AERUGINOSA 1.5 X-RAY DIFFRACTION 100.0
4XFD LIGASE 12/26/14 Crystal Structure of a NH(3)-dependent NAD(+) synthetase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.55 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004537 (547 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA4920
Search term: nadE

Human Homologs


The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase.
Willison JC, Tissot G
J. Bacteriol. 1994 Jun;176(11):3400-2
PubMed ID: 8195100
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep;277(5331):1453-62
PubMed ID: 9278503
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142