Pseudomonas aeruginosa PAO1, PA4931 (dnaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4931
Name
dnaB
Replicon chromosome
Genomic location 5534162 - 5535556 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253618.1
GI 15600124
Affymetrix PA4931_dnaB_at
Entrez 879625
GenBank AAG08316.1
INSDC AAG08316.1
NCBI Locus Tag PA4931
protein_id(GenBank) gb|AAG08316.1|AE004906_4|gnl|PseudoCAP|PA4931
TIGR NTL03PA04932
UniParc UPI00000C6EA6
UniProtKB Acc Q9HUN3
UniProtKB ID Q9HUN3_PSEAE
UniRef100 UniRef100_Q9HUN3
UniRef50 UniRef50_P0ACB0
UniRef90 UniRef90_A0A024HPK3

Product

Feature Type CDS
Coding Frame 1
Product Name
replicative DNA helicase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.55
Kyte-Doolittle Hydrophobicity Value -0.294
Molecular Weight (kDa) 51.6
Isoelectric Point (pI) 4.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004546 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4931
Search term: dnaB

Human Homologs

References

Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.
Nakayama N, Arai N, Bond MW, Kaziro Y, Arai K
J Biol Chem 1984 Jan 10;259(1):97-101
PubMed ID: 6323420
DnaB helicase is unable to dissociate RNA-DNA hybrids. Its implication in the polar pausing of replication forks at ColE1 origins.
Santamaría D, de la Cueva G, Martínez-Robles ML, Krimer DB, Hernández P, Schvartzman JB
J Biol Chem 1998 Dec 11;273(50):33386-96
PubMed ID: 9837915