Pseudomonas aeruginosa PAO1, PA4951 (orn)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4951
Name
orn
Replicon chromosome
Genomic location 5556281 - 5556823 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253638.1
GI 15600144
Affymetrix PA4951_orn_at
Entrez 878205
GenBank AAG08336.1
INSDC AAG08336.1
NCBI Locus Tag PA4951
protein_id(GenBank) gb|AAG08336.1|AE004908_2|gnl|PseudoCAP|PA4951
TIGR NTL03PA04952
UniParc UPI0000130E89
UniProtKB Acc P57665
UniProtKB ID ORN_PSEAE
UniRef100 UniRef100_Q02F67
UniRef50 UniRef50_P45340
UniRef90 UniRef90_Q02F67

Product

Feature Type CDS
Coding Frame 1
Product Name
oligoribonuclease
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.38
Kyte-Doolittle Hydrophobicity Value -0.494
Molecular Weight (kDa) 20.8
Isoelectric Point (pI) 5.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7VA2 HYDROLASE 08/27/21 PaOrn Oligoribonuclease D11A mutant with product GMP complex structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.3 X-RAY DIFFRACTION 99.4
7V9Z HYDROLASE 08/27/21 PaOrn Oligoribonuclease native structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.85 X-RAY DIFFRACTION 100.0
7VA3 HYDROLASE 08/27/21 PaOrn Oligoribonuclease D11A mutant with substrate pGpG complex structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 1.8 X-RAY DIFFRACTION 99.4
7VA6 HYDROLASE 08/27/21 PaOrn Oligoribonuclease D11A mutant with RNA GU complex structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 2.1 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004566 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4951
Search term: orn
Search term: oligoribonuclease

Human Homologs

Ensembl 110, assembly GRCh38.p14
RNA exonuclease 2 [Source:HGNC Symbol;Acc:HGNC:17851]
E-value: 3.9e-47
Percent Identity: 50.6

References

Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily.
Zhang X, Zhu L, Deutscher MP
J Bacteriol 1998 May;180(10):2779-81
PubMed ID: 9573169
Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa.
Cohen D, Mechold U, Nevenzal H, Yarmiyhu Y, Randall TE, Bay DC, Rich JD, Parsek MR, Kaever V, Harrison JJ, Banin E
Proc Natl Acad Sci U S A 2015 Sep 8;112(36):11359-64
PubMed ID: 26305928