Pseudomonas aeruginosa PAO1, PA4973 (thiC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4973
Name
thiC
Replicon chromosome
Genomic location 5581762 - 5583645 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253660.1
GI 15600166
Affymetrix PA4973_thiC_at
Entrez 879702
GenBank AAG08358.1
INSDC AAG08358.1
NCBI Locus Tag PA4973
protein_id(GenBank) gb|AAG08358.1|AE004910_5|gnl|PseudoCAP|PA4973
TIGR NTL03PA04974
UniParc UPI0000136DA8
UniProtKB Acc Q9HUJ2
UniProtKB ID THIC_PSEAE
UniRef100 UniRef100_B7V362
UniRef50 UniRef50_Q9PC93
UniRef90 UniRef90_B7V362

Product

Feature Type CDS
Coding Frame 1
Product Name
thiamin biosynthesis protein ThiC
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.66
Kyte-Doolittle Hydrophobicity Value -0.364
Molecular Weight (kDa) 69.8
Isoelectric Point (pI) 5.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 485 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004588 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4973
Search term: thiC

Human Homologs

References

Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.
Vander Horn PB, Backstrom AD, Stewart V, Begley TP
J Bacteriol 1993 Feb;175(4):982-92
PubMed ID: 8432721
Expression of thiamin biosynthetic genes (thiCOGE) and production of symbiotic terminal oxidase cbb3 in Rhizobium etli.
Miranda-Ríos J, Morera C, Taboada H, Dávalos A, Encarnación S, Mora J, Soberón M
J Bacteriol 1997 Nov;179(22):6887-93
PubMed ID: 9371431