Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5013
|
Name |
ilvE
|
Replicon | chromosome |
Genomic location | 5631947 - 5632870 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253700.1 |
GI | 15600206 |
Affymetrix | PA5013_ilvE_at |
Entrez | 881423 |
GenBank | AAG08398.1 |
INSDC | AAG08398.1 |
NCBI Locus Tag | PA5013 |
protein_id(GenBank) | gb|AAG08398.1|AE004913_13|gnl|PseudoCAP|PA5013 |
TIGR | NTL03PA05014 |
UniParc | UPI000012D5A1 |
UniProtKB Acc | O86428 |
UniProtKB ID | ILVE_PSEAE |
UniRef100 | UniRef100_O86428 |
UniRef50 | UniRef50_O27481 |
UniRef90 | UniRef90_O86428 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
branched-chain amino acid transferase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -4.62 |
Kyte-Doolittle Hydrophobicity Value | -0.220 |
Molecular Weight (kDa) | 34.1 |
Isoelectric Point (pI) | 6.19 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6NST | TRANSFERASE | 01/25/19 | Crystal structure of branched chain amino acid aminotransferase from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.136 | X-RAY DIFFRACTION | 99.7 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004620 (361 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5013
Search term: ilvE
Search term: branched-chain amino acid transferase
|
Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence.
Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H
J Biochem 1985 Apr;97(4):993-9
PubMed ID: 3897211
|
Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties.
Inoue K, Kuramitsu S, Aki K, Watanabe Y, Takagi T, Nishigai M, Ikai A, Kagamiyama H
J Biochem 1988 Nov;104(5):777-84
PubMed ID: 3069843
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|