Pseudomonas aeruginosa PAO1, PA5013 (ilvE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5013
Name
ilvE
Replicon chromosome
Genomic location 5631947 - 5632870 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253700.1
GI 15600206
Affymetrix PA5013_ilvE_at
Entrez 881423
GenBank AAG08398.1
INSDC AAG08398.1
NCBI Locus Tag PA5013
protein_id(GenBank) gb|AAG08398.1|AE004913_13|gnl|PseudoCAP|PA5013
TIGR NTL03PA05014
UniParc UPI000012D5A1
UniProtKB Acc O86428
UniProtKB ID ILVE_PSEAE
UniRef100 UniRef100_O86428
UniRef50 UniRef50_O27481
UniRef90 UniRef90_O86428

Product

Feature Type CDS
Coding Frame 1
Product Name
branched-chain amino acid transferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.62
Kyte-Doolittle Hydrophobicity Value -0.220
Molecular Weight (kDa) 34.1
Isoelectric Point (pI) 6.19

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6NST TRANSFERASE 01/25/19 Crystal structure of branched chain amino acid aminotransferase from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.136 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004620 (361 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5013
Search term: ilvE

Human Homologs

References

Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence.
Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H
J Biochem 1985 Apr;97(4):993-9
PubMed ID: 3897211
Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties.
Inoue K, Kuramitsu S, Aki K, Watanabe Y, Takagi T, Nishigai M, Ikai A, Kagamiyama H
J Biochem 1988 Nov;104(5):777-84
PubMed ID: 3069843
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994