Pseudomonas aeruginosa PAO1, PA5034 (hemE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5034
Name
hemE
Replicon chromosome
Genomic location 5664990 - 5666057 (- strand)

Cross-References

RefSeq NP_253721.1
GI 15600227
Affymetrix PA5034_hemE_at
DNASU PaCD00008169
Entrez 881202
GenBank AAG08419.1
INSDC AAB39261.1
NCBI Locus Tag PA5034
protein_id(GenBank) gb|AAG08419.1|AE004916_8|gnl|PseudoCAP|PA5034
TIGR NTL03PA05035
UniParc UPI0000129058
UniProtKB Acc P95458
UniProtKB ID DCUP_PSEAE
UniRef100 UniRef100_P95458
UniRef50 UniRef50_Q7NZ00
UniRef90 UniRef90_P95458

Product

Feature Type CDS
Coding Frame 1
Product Name
uroporphyrinogen decarboxylase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.17
Kyte-Doolittle Hydrophobicity Value -0.069
Molecular Weight (kDa) 38.8
Isoelectric Point (pI) 6.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WSH LYASE 10/27/14 Crystal structure of Probable Uroporphyrinogen decarboxylase (UPD) (URO-D) from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 480 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004639 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5034
Search term: hemE

Human Homologs

Ensembl 110, assembly GRCh38.p14
uroporphyrinogen decarboxylase [Source:HGNC Symbol;Acc:HGNC:12591]
E-value: 4.0e-88
Percent Identity: 49.3

References

Characterization of a hemA/hemE mutant of E. coli and regulation of hemE.
Pido S, Tsoi KW, Umanoff H, Cosloy SD, Russell CS
Cell Mol Biol (Noisy-le-grand) 1994 Nov;40(7):945-56
PubMed ID: 7849561