Pseudomonas aeruginosa PAO1, PA5044 (pilM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5044
Name
pilM
Replicon chromosome
Genomic location 5679649 - 5680713 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253731.1
GI 15600237
Affymetrix PA5044_pilM_at
Entrez 881162
GenBank AAG08429.1
INSDC AAG08429.1
NCBI Locus Tag PA5044
protein_id(GenBank) gb|AAG08429.1|AE004917_8|gnl|PseudoCAP|PA5044
TIGR NTL03PA05045
UniParc UPI00000D4249
UniProtKB Acc G3XD28
UniProtKB ID G3XD28_PSEAE
UniRef100 UniRef100_W0Z0P4
UniRef50 UniRef50_A0A078M0F9
UniRef90 UniRef90_A6VDG5

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilM
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.81
Kyte-Doolittle Hydrophobicity Value 0.085
Molecular Weight (kDa) 38.0
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5EOY PEPTIDE BINDING PROTEIN 11/10/15 Pseudomonas aeruginosa SeMet-PilM bound to ADP Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 100.0
5EQ6 PEPTIDE BINDING PROTEIN 11/12/15 Pseudomonas aeruginosa PilM bound to AMP-PNP Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 3.5 X-RAY DIFFRACTION 100.0
5EOX PEPTIDE BINDING PROTEIN 11/10/15 Pseudomonas aeruginosa PilM bound to ADP Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.4 X-RAY DIFFRACTION 100.0
5EOU PEPTIDE BINDING PROTEIN 11/10/15 Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.4 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004647 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5044
Search term: pilM

Human Homologs

References

Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Watson AA, McCaul TF, Mattick JS
Mol Microbiol 1995 May;16(3):497-508
PubMed ID: 7565110
Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.
Roine E, Nunn DN, Paulin L, Romantschuk M
J Bacteriol 1996 Jan;178(2):410-7
PubMed ID: 8550460
PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin.
Ayers M, Sampaleanu LM, Tammam S, Koo J, Harvey H, Howell PL, Burrows LL
J Mol Biol 2009 Nov 20;394(1):128-42
PubMed ID: 19857645