Pseudomonas aeruginosa PAO1, PA5112 (estA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5112
Name
estA
Replicon chromosome
Genomic location 5754298 - 5756238 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_253799.1
GI 15600305
Affymetrix PA5112_estA_at
Entrez 878826
GenBank AAG08497.1
INSDC AAG08497.1
NCBI Locus Tag PA5112
protein_id(GenBank) gb|AAG08497.1|AE004924_2|gnl|PseudoCAP|PA5112
TIGR NTL03PA05113
UniParc UPI00000D426D
UniProtKB Acc O33407
UniProtKB ID ESTA_PSEAE
UniRef100 UniRef100_O33407
UniRef50 UniRef50_O33407
UniRef90 UniRef90_O33407

Product

Feature Type CDS
Coding Frame 1
Product Name
esterase EstA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (69.61 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -21.63
Kyte-Doolittle Hydrophobicity Value -0.375
Molecular Weight (kDa) 69.6
Isoelectric Point (pI) 4.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3KVN HYDROLASE 11/30/09 Crystal structure of the full-length autotransporter EstA from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.499 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
(Hydroxyethyloxy)Tri(Ethyloxy)Octane DB04233 DrugBank 5.1.4 O33407 Esterase EstA 100.0 646 0.0e+00

Virulence Evidence

Source 1
Database Victors : 4222
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 92 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004538 (814 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5112
Search term: estA
Search term: esterase EstA

Human Homologs

References

A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa.
Wilhelm S, Tommassen J, Jaeger KE
J Bacteriol 1999 Nov;181(22):6977-86
PubMed ID: 10559163
Evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: DNA sequences and characterization of Pseudomonas putida trpE and trpGDC.
Essar DW, Eberly L, Crawford IP
J Bacteriol 1990 Feb;172(2):867-83
PubMed ID: 2404959
Extracellular enzymes affect biofilm formation of mucoid Pseudomonas aeruginosa.
Tielen P, Rosenau F, Wilhelm S, Jaeger KE, Flemming HC, Wingender J
Microbiology (Reading) 2010 Jul;156(Pt 7):2239-2252
PubMed ID: 20360178
The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation.
Wilhelm S, Gdynia A, Tielen P, Rosenau F, Jaeger KE
J Bacteriol 2007 Sep;189(18):6695-703
PubMed ID: 17631636
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878