Pseudomonas aeruginosa PAO1, PA5129 (grxC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: grx
Replicon chromosome
Genomic location 5777134 - 5777388 (- strand)
Transposon Mutants 1 transposon mutants in PAO1


RefSeq NP_253816.1
GI 15600322
Affymetrix PA5129_grx_at
DNASU PaCD00008207
Entrez 877828
GenBank AAG08514.1
INSDC AAG08514.1
NCBI Locus Tag PA5129
protein_id(GenBank) gb|AAG08514.1|AE004926_4|gnl|PseudoCAP|PA5129
UniParc UPI000012B7D6
UniProtKB Acc Q9HU55
UniRef100 UniRef100_Q9HU55
UniRef50 UniRef50_P0AC64
UniRef90 UniRef90_Q9HU55


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 2
Synonyms glutaredoxin
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.34
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 9.2
Isoelectric Point (pI) 7.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 317 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004722 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5129
Search term: grxC
Search term: GrxC

Human Homologs


Two additional glutaredoxins exist in Escherichia coli: glutaredoxin 3 is a hydrogen donor for ribonucleotide reductase in a thioredoxin/glutaredoxin 1 double mutant.
Aslund F, Ehn B, Miranda-Vizuete A, Pueyo C, Holmgren A
Proc Natl Acad Sci U S A 1994 Oct 11;91(21):9813-7
PubMed ID: 7937896
Glutaredoxin-3 from Escherichia coli. Amino acid sequence, 1H AND 15N NMR assignments, and structural analysis.
Aslund F, Nordstrand K, Berndt KD, Nikkola M, Bergman T, Ponstingl H, Jörnvall H, Otting G, Holmgren A
J Biol Chem 1996 Mar 22;271(12):6736-45
PubMed ID: 8636094
NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: implications for the enzymatic mechanism.
Nordstrand K, slund F, Holmgren A, Otting G, Berndt KD
J Mol Biol 1999 Feb 19;286(2):541-52
PubMed ID: 9973569
Mutation of the gene encoding monothiol glutaredoxin (GrxD) in Pseudomonas aeruginosa increases its susceptibility to polymyxins.
Romsang A, Leesukon P, Duangnkern J, Vattanaviboon P, Mongkolsuk S
Int J Antimicrob Agents 2015 Mar;45(3):314-8
PubMed ID: 25593012