Pseudomonas aeruginosa PAO1, PA5172 (arcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5172
Name
arcB
Replicon chromosome
Genomic location 5823716 - 5824726 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253859.1
GI 15600365
Affymetrix PA5172_arcB_at
Entrez 881792
GenBank AAG08557.1
INSDC AAG08557.1
NCBI Locus Tag PA5172
protein_id(GenBank) gb|AAG08557.1|AE004930_3|gnl|PseudoCAP|PA5172
TIGR NTL03PA05173
UniParc UPI0000165EDA
UniProtKB Acc P08308
UniProtKB ID OTCC_PSEAE
UniRef100 UniRef100_P08308
UniRef50 UniRef50_P08308
UniRef90 UniRef90_P08308

Product

Feature Type CDS
Coding Frame 1
Product Name
ornithine carbamoyltransferase, catabolic
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (38.11 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.96
Kyte-Doolittle Hydrophobicity Value -0.404
Molecular Weight (kDa) 38.1
Isoelectric Point (pI) 6.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1DXH TRANSFERASE 01/05/00 Catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0
1ORT TRANSFERASE 08/24/95 ORNITHINE TRANSCARBAMOYLASE FROM PSEUDOMONAS AERUGINOSA Pseudomonas aeruginosa 3 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 644 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004760 (492 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5172
Search term: arcB

Human Homologs

Ensembl 110, assembly GRCh38.p14
ornithine transcarbamylase [Source:HGNC Symbol;Acc:HGNC:8512]
E-value: 3.4e-52
Percent Identity: 38.0

References

Primary and quaternary structure of the catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa. Extensive sequence homology with the anabolic ornithine carbamoyltransferases of Escherichia coli.
Baur H, Stalon V, Falmagne P, Luethi E, Haas D
Eur J Biochem 1987 Jul 1;166(1):111-7
PubMed ID: 3109911
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344