Pseudomonas aeruginosa PAO1, PA5214 (gcvH1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5214
Name
gcvH1
Replicon chromosome
Genomic location 5871231 - 5871620 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253901.1
GI 15600407
Affymetrix PA5214_gcvH1_at
Entrez 880637
GenBank AAG08599.1
INSDC AAG08599.1
NCBI Locus Tag PA5214
protein_id(GenBank) gb|AAG08599.1|AE004934_6|gnl|PseudoCAP|PA5214
TIGR NTL03PA05215
UniParc UPI000012B30E
UniProtKB Acc Q9HTX6
UniProtKB ID GCSH2_PSEAE
UniRef100 UniRef100_Q9HTX6
UniRef50 UniRef50_Q887L4
UniRef90 UniRef90_Q9HTX6

Product

Feature Type CDS
Coding Frame 1
Product Name
glycine cleavage system protein H1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -15.52
Kyte-Doolittle Hydrophobicity Value -0.098
Molecular Weight (kDa) 13.6
Isoelectric Point (pI) 3.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 512 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004796 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5214
Search term: gcvH1

Human Homologs

Ensembl 110, assembly GRCh38.p14
glycine cleavage system protein H [Source:HGNC Symbol;Acc:HGNC:4208]
E-value: 1.1e-24
Percent Identity: 51.3
Ensembl 110, assembly GRCh38.p14
glycine cleavage system protein H [Source:HGNC Symbol;Acc:HGNC:4208]
E-value: 1.1e-24
Percent Identity: 51.3

References

Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system.
Okamura-Ikeda K, Ohmura Y, Fujiwara K, Motokawa Y
Eur J Biochem 1993 Sep 1;216(2):539-48
PubMed ID: 8375392
The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system.
Stauffer LT, Ghrist A, Stauffer GV
DNA Seq 1993;3(6):339-46
PubMed ID: 8219277