Pseudomonas aeruginosa PAO1, PA5312 (pauC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5312
Name
pauC
Synonym: kauB
Replicon chromosome
Genomic location 5982097 - 5983590 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253999.1
GI 15600505
Affymetrix PA5312_at
Entrez 877979
GenBank AAG08697.1
INSDC AAG08697.1
NCBI Locus Tag PA5312
protein_id(GenBank) gb|AAG08697.1|AE004944_1|gnl|PseudoCAP|PA5312
TIGR NTL03PA05313
UniParc UPI00000C5F9F
UniProtKB Acc Q9HTP2
UniProtKB ID Q9HTP2_PSEAE
UniRef100 UniRef100_W0YZ73
UniRef50 UniRef50_P23883
UniRef90 UniRef90_V6ANW6

Product

Feature Type CDS
Coding Frame 1
Product Name
Aldehyde dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.41
Kyte-Doolittle Hydrophobicity Value -0.025
Molecular Weight (kDa) 53.1
Isoelectric Point (pI) 5.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6B4R OXIDOREDUCTASE 09/27/17 The crystal structure of the aldehyde dehydrogenase KauB from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.55 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database Victors : 4212
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 600 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004884 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5312
Search term: pauC

Human Homologs

Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 1 family member B1 [Source:HGNC Symbol;Acc:HGNC:407]
E-value: 3.9e-113
Percent Identity: 46.2

References

Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.
Yao X, He W, Lu CD
J. Bacteriol. 2011 Aug;193(15):3923-30
PubMed ID: 21622750
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994