Pseudomonas aeruginosa PAO1, PA5320 (coaC)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5320
Name	coaC Synonym: coaB Synonym: dfp Synonym: coaBC
Replicon	chromosome
Genomic location	5989460 - 5990668 (+ strand)

Cross-References

RefSeq	NP_254007.1
GI	15600513
Affymetrix	PA5320_dfp_at
DNASU	PaCD00008297
Entrez	877887
GenBank	AAG08705.1
INSDC	AAG08705.1
NCBI Locus Tag	PA5320
protein_id(GenBank)	gb|AAG08705.1|AE004944_9|gnl|PseudoCAP|PA5320
TIGR	NTL03PA05321
UniParc	UPI00000C5FA4
UniProtKB Acc	Q9HTN4
UniProtKB ID	Q9HTN4_PSEAE
UniRef100	UniRef100_Q9HTN4
UniRef50	UniRef50_P0ABQ1
UniRef90	UniRef90_A6VEC7

Product

Feature Type	CDS
Coding Frame	1
Product Name	Phosphopantothenoylcysteine synthase/(R)-4'-phospho-N-pantothenoylcysteine decarboxylase Product Name Confidence: Class 2
Synonyms	CoaBC protein Dfp protein
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-3.94
Kyte-Doolittle Hydrophobicity Value	0.022
Molecular Weight (kDa)	43.1
Isoelectric Point (pI)	6.25

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 632 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004891 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5320 Search term: coaC Search term: Phosphopantothenoylcysteine synthase/(R)-4'-phospho-N-pantothenoylcysteine decarboxylase

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000343190 phosphopantothenoylcysteine decarboxylase [Source:HGNC Symbol;Acc:HGNC:28107] E-value: 3.6e-16 Percent Identity: 31.6

References

Molecular characterization of lantibiotic-synthesizing enzyme EpiD reveals a function for bacterial Dfp proteins in coenzyme A biosynthesis. Kupke T, Uebele M, Schmid D, Jung G, Blaesse M, Steinbacher S J Biol Chem 2000 Oct 13;275(41):31838-46 PubMed ID: 10922366

Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria. Strauss E, Kinsland C, Ge Y, McLafferty FW, Begley TP J Biol Chem 2001 Apr 27;276(17):13513-6 PubMed ID: 11278255

dfp Gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein. Spitzer ED, Weiss B J Bacteriol 1985 Dec;164(3):994-1003 PubMed ID: 2999089

beta-Alanine auxotrophy associated with dfp, a locus affecting DNA synthesis in Escherichia coli. Spitzer ED, Jimenez-Billini HE, Weiss B J Bacteriol 1988 Feb;170(2):872-6 PubMed ID: 3123465