Pseudomonas aeruginosa PAO1, PA5336 (gmk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5336
Name
gmk
Replicon chromosome
Genomic location 6005199 - 6005810 (+ strand)

Cross-References

RefSeq NP_254023.1
GI 15600529
Affymetrix PA5336_gmk_at
DNASU PaCD00008306
Entrez 878047
GenBank AAG08721.1
INSDC AAG08721.1
NCBI Locus Tag PA5336
protein_id(GenBank) gb|AAG08721.1|AE004946_5|gnl|PseudoCAP|PA5336
TIGR NTL03PA05337
UniParc UPI000012DDEE
UniProtKB Acc Q9HTM2
UniProtKB ID KGUA_PSEAE
UniRef100 UniRef100_Q9HTM2
UniRef50 UniRef50_Q9HTM2
UniRef90 UniRef90_Q9HTM2

Product

Feature Type CDS
Coding Frame 1
Product Name
guanylate kinase
Product Name Confidence: Class 2
Synonyms GMP kinase
Evidence for Translation
Charge (pH 7) -6.31
Kyte-Doolittle Hydrophobicity Value -0.406
Molecular Weight (kDa) 23.1
Isoelectric Point (pI) 5.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8EGL TRANSFERASE 09/12/22 Crystal Structure of Guanylate kinase from Pseudomonas aeruginosa PAO1 in complex with GMP and ADP Pseudomonas aeruginosa PAO1 2.35 X-RAY DIFFRACTION 100.0
7U5F TRANSFERASE 03/02/22 Crystal structure of guanylate kinase from Pseudomonas aeruginosa PAO1 in complex with GMP Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004905 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5336
Search term: gmk
Search term: guanylate kinase

Human Homologs

Ensembl 110, assembly GRCh38.p14
guanylate kinase 1 [Source:HGNC Symbol;Acc:HGNC:4693]
E-value: 1.1e-24
Percent Identity: 37.7
Ensembl 110, assembly GRCh38.p14
guanylate kinase 1 [Source:HGNC Symbol;Acc:HGNC:4693]
E-value: 1.1e-24
Percent Identity: 37.7

References

Guanylate kinase of Escherichia coli K-12.
Gentry D, Bengra C, Ikehara K, Cashel M
J Biol Chem 1993 Jul 5;268(19):14316-21
PubMed ID: 8390989